Unlock instant, AI-driven research and patent intelligence for your innovation.
Quick bacterium identification kit for sputum sample and detection method for the same
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A detection method and kit technology, applied in the field of clinical microbial identification, to achieve the effect of convenient operation, low cost and strong specificity
Active Publication Date: 2010-10-20
南京迪安医学检验所有限公司
View PDF1 Cites 7 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
So far, there have been no products and reports of sequencing to distinguish pathogenic bacteria in sputum samples at home and abroad. Therefore, clinically, if you want to quickly obtain information on bacterial infections in sputum samples, you need to wait 3-7 days or longer
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0064] Embodiment 1: the preparation method of kit.
[0065] (1) Liquefactionreagent: 0.2M NaOH, purchased from Shanghai Shisihewei Chemical Co., Ltd.
[0066] (2) Washing reagent: 0.9% NaCl solution, purchased from Shanghai Shisi Hewei Chemical Co., Ltd.
[0068] 5% (w / v) g / mL Chelex 100 buffer: containing 0.03% (w / v) g / mL SDS (purchased from Hangzhou FluorideBiotechnology Co., Ltd.), 1% (v / v) Tween-20 ( Purchased from Sigma Corporation in the U.S.), 1% (v / v) NP-40 (purchased from Sigma Corporation in the U.S.),
[0069] 20 mg / mL proteinase K: (purchased from Amresco).
[0070] (4) Reaction solution:
[0071] PCR Buffer: 0.1% (v / v) NP-40, 0.02% (v / v) gelatin (purchased from Sigma, USA), 0.06% (w / v) g / mL BSA (purchased from Sigma, USA), 0.1 %(v / v) Tween-20 (purchased from Sigma, USA), 0.06MpH8.9Tricine (purchased from Merck),
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to the field of clinical microbiological assay and provides a quick bacterium identification kit for a sputum sample and a detection method for the same. The kit comprises two universal primers for propagating the V1 variable regions of the 16S rRNA of a bacterium, two specific primers for shielding the variable regions of the 16S rRNA of a neisseria bacterium, two specific primers for shielding the variable regions of the 16S rRNA of a veillonella parvula, a sequencing primer for sequencing the positive segment of the V1, and an avidin-marked magnetic ball, wherein the sequencing result is compared with a database to determine the species of the bacteria. By applying the kit, the bacteria in a sputum sample can be distinguished by once sequencing, thus, the kit can be applied in the clinical diagnosis on the bacterial infection in the sputum sample. The invention wins precious rescuing time for a clinical treatment and has the advantages of low cost, convenient operation and high specificity.
Description
technical field [0001] The invention belongs to the technical field of clinical microorganism identification, and in particular relates to a kit and a detection method for rapidly identifying pathogenic bacteria in sputum samples. Background technique [0002] Traditional bacterial culture and biochemical analysis can no longer satisfy the diagnosis and epidemiological research of various pathogenic microorganisms. Researchers not only hope to study various bacteria and viruses at the molecular level, but also hope that the detection method will be faster and more accurate. The throughput is higher, and human influence factors should be reduced as much as possible to facilitate the construction of standardized operating procedures. PCR and quantitative PCR methods cannot obtain the gold standard of molecular diagnosis - gene sequence, and are limited by the detection of bacterial species. Each bacterial species requires specific PCR primers and or probes, which greatly limit...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More