Method of preparing lead citrate staining solution for electron microscope experiment

Abstract

The invention discloses a method of preparing lead citrate staining solution for electron microscope experiments, specifically a method for industrializing lead citrate staining solution for electron microscope experiments. The method comprises the following steps of: adding 3.52g of sodium citrate Na3(C6H5O7).2H2O, 2.66g of lead citrate to 16mmol of sodium hydroxide, and adding distilled water to 100ml; keeping in a dark place, closely stirring for 12h (or treating by ultrasonic for 2h), and purifying; testing the pH value till pH=12+ / -0.5; filtering through a filter bulb and filtering through a filter film; testing the clarity: no visible particle impurity; washing in a brown ampoule and drying; packaging, charging nitrogen, and sealing by fusing through a fusing sealer; testing in light: observing the product sealed by fusing in the light at a black background, removing the unaccepted products with bubble head, shrunken head, carbonized point and the like; printing and packaging; and testing the finished products. The invention has the advantages of high quality, low cost, safety, good environment friendliness, long service life and the like.

Description

technical field [0001] The invention relates to a method for preparing a lead citrate staining solution for electron microscope experiments, more specifically a method for commercializing the lead citrate staining solution for electron microscope experiments. Background technique [0002] At present, the lead citrate staining solution used in electron microscopy experiments is mostly prepared by the laboratory, that is, the raw materials are purchased and configured according to the experimental needs. The configuration method is as follows: the reagent uses lead nitrate and sodium citrate as raw materials to prepare a sodium citrate milky white suspension. Finally, add sodium hydroxide to make it a clear solution; since lead citrate is easily transformed into lead carbonate precipitation when it encounters carbon dioxide in the air, it should be stored and handled to avoid contact with air as much as possible. There are following unfavorable conditions in the whole preparat...

AI Technical Summary

Problems solved by technology

There are following unfavorable situations in the whole preparation process: 1. The amount of experiment is small. As a sample, only 0.2ml of prepared reagent is needed, and each preparation generally needs 50ml, so 99% of the raw materials will remain, resulting in unnecessary experiments Waste of cost; 2. The remaining reagents can only be stored in the reagent bottle for a few months before precipitation and failure. In terms of waste liquid treatment, it will cause new cost expenditures, potential safety hazards, waste of resources and environmental pollution to the experimental unit; 3. In artificial On the one hand, it takes about 2 days for the self-prepared reagent to be cleaned from the container, dried, weighed, and prepared; the heavy metals in the reagent during the preparation process will also cause adverse effects on the health of the preparer