Real-time fluorescent RT-PCR method for detecting whether arabis mosaic virus, bean yellow mosaic virus and lily symptomless virus infect host

Abstract

The invention discloses a real-time fluorescent RT-PCR method for detecting whether arabis mosaic virus, bean yellow mosaic virus and lily symptomless virus infect a host. In the invention, according to the RNAs of the arabis mosaic virus, the bean yellow mosaic virus and the lily symptomless virus, a specific amplimer and a fluorescent probe are designed, RT-Realt time PCR reaction is carried out the RNA template of virus to be identified by means of the specific amplimer and the fluorescent probe, and whether the arabis mosaic virus, the bean yellow the mosaic virus and the lily symptomless virus infect the host is rapidly detected by carrying out real-time fluorescent detection on RT-Real time PCR products.

Description

technical field [0001] The invention relates to a virus species detection technology, in particular to a rapid detection technology for whether Arabidopsis thaliana mosaic virus, bean yellow mosaic virus and lily asymptomatic virus infect hosts. Background technique [0002] Arabis mosaic virus (ArMV), Bean yellow mosaic virus (BYMV) and Lily symptomless virus (LSV) are the main pathogens infecting lily and gladiolus crops, causing greater economic losses to growers. Arabidopsis mosaic virus has a wide range of hosts and can infect about 174 genera and 215 species of plants. It naturally infects and harms a variety of flowers, melons, beans and fruit trees, causing mosaic, yellowing, chlorosis, dwarfing, and shrinking Even symptoms such as necrosis are the entry plant quarantine pests stipulated by our country. BYMV has a wide range of hosts, and the main hosts are ornamental plants such as gladiolus and leguminous plants. The growth potential of gladiolus seedlings infec...

AI Technical Summary

Problems solved by technology

The growth potential of gladiolus seedlings infected by BYMV was weakened, white spots appeared on the leaves, and the flowers were variegated, resulting in a decrease in the quality of cut flowers and a decrease in bulb yield. Morbidity can be as high as 67% to 100%, resulting in 59% to 96% yield loss

The infection rate of lily by LSV is as high as 73%. When it is infected alone, it has no obvious symptoms. Under natural conditions, it is often co-infected with other viruses, resulting in serious mosaic leaves and dwarfing of the whole plant, affecting the yield of lily. and business value

[0003] The detection of Arabidopsis mosaic virus, lily asymptomatic virus and bean yellow mosaic virus can be detected by conventional enzyme-linked immunosorbent assay (ELISA), but this detection method has low sensitivity and bias; especially when gladiola and lily are in In the dormant state, the virus concentration in the bulb is low, and it cannot be detected by conventional enzyme-linked immunosorbent assay (ELISA)

Deng Congliang et al. designed specific detection primers and probes based on the conserved sequences of the coat protein genes of different ArMV isolates, and established a real-time RT-PCR detection method for the detection of ArMV, such as Chinese patent CN1952649A, which effectively solved the enzyme-linked immunosorbent assay method. (ELISA) technical defect of low sensitivity

[0004] When the staff used real-time RT-PCR detection technology to detect the three main pathogens (Arabidopsis thaliana mosaic virus, bean yellow mosaic virus and lily asymptomatic virus) that infect lily and gladiolus, three detection experiments were required, and the speed was slow. , so it is necessary to improve