Method for producing ethanol by fermenting sweet sorghum juice through yeast refluxing method
A technology of sweet sorghum and reflux method, which is applied in the field of producing fuel ethanol by fermenting sweet sorghum sugar juice with yeast reflux method, which can solve the problems of high raw material consumption per ton of ethanol, unsuitable for repeated use, and large amount of yeast, etc., and achieves less sugar consumption, Suitable for large-scale industrial production, the effect of reducing the amount of yeast
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Embodiment 1
[0034] Sweet sorghum sugar juice raw material liquid total sugar 40.75% (sucrose 23.95%, glucose 7.63%, fructose 9.17%), be mixed with the sweet sorghum sugar liquid of total sugar 23.43g / 100ml with sterilized domestic drinking water, pH is 4 .
[0035] Weigh 20 g of yeast (CGMCC No.3889), add 200 ml of water, incubate in a water bath at 35°C for 5 minutes, and then activate at 30°C for 15 minutes for later use.
[0036] (1) Pre-fermentation: add the recycled yeast after soaking and sterilization into the sterilized fermenter, and adjust the amount of yeast in the fermenter with newly activated yeast so that the yeast concentration is not less than 400 million / ml, add sweet sorghum sugar liquid (23.63g / 100ml sugar solution), the speed is 150L / h, and the air flow rate is 500L / min. Turn on the temperature control program at 15 minutes, the temperature is controlled at 32.5°C, and the gas flow rate is adjusted to 750L / min. Start stirring at 20 minutes, the speed is 100rpm;
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Embodiment 2
[0049] Sweet sorghum juice raw material liquid total sugar 38.61% (sucrose 20.69%, glucose 8.23%, fructose 9.69%), be mixed with the sweet sorghum sugar liquid of total sugar 23.76g / 100ml, pH is 4.
[0050] Weigh 25 g of yeast (CGMCC No. 3889), add 200 ml of water, incubate in a water bath at 35°C for 5 minutes, and then activate at 30°C for 15 minutes for later use.
[0051] (1) Pre-fermentation: add the recycled yeast after soaking and sterilization into the sterilized fermenter, and adjust the amount of yeast in the fermenter with newly activated yeast so that the yeast concentration is not less than 400 million / ml, add sweet sorghum sugar liquid (23.76g / 100ml sugar solution), the speed is 180L / h, and the air flow rate is 500L / min. Turn on the temperature control program at 15 minutes, the temperature is controlled at 32.5°C, and the gas flow rate is adjusted to 780L / min. Start stirring at 20 minutes, the speed is 100rpm;
[0052] (2) Fermentation: continue to add sweet s...
Embodiment 3
[0063] Sweet sorghum juice raw material liquid total sugar 40.75% (sucrose 23.95%, glucose 7.63%, fructose 9.17%), be mixed with the sweet sorghum sugar liquid of total sugar 24.14g / 100ml, pH is 4.
[0064] Weigh 30 g of yeast (CGMCC No.3889), add 200 ml of water, incubate in a 35°C water bath for 5 minutes, and then activate at 30°C for 15 minutes for later use.
[0065] (1) Pre-fermentation: Add the activated yeast into the sterilized fermenter, the yeast concentration is not less than 500 million / ml, add sweet sorghum sugar solution (24.14g / 100ml sugar solution), the speed is 200L / h , Air flow 550L / min. Turn on the temperature control program at 15 minutes, the temperature is controlled at 32.5°C, and the gas flow rate is adjusted to 800L / min. Start stirring at 25 minutes, the speed is 100rpm;
[0066] (2) Fermentation: Continue to add sweet sorghum sugar solution (24.14g / 100ml sugar solution), the speed is 180L / h, the air flow rate is increased from 800L / min to 1000L / min...
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