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Method for preparing sucrose-6-acetic ester by enzymatic method

A technology of acetate and vinyl acetate, applied in the field of biocatalysis, can solve the problems of low conversion rate, large enzyme dosage, long reaction time, etc., and achieve the effects of high conversion rate, short reaction time and product safety

Inactive Publication Date: 2010-11-17
HANGZHOU XINFU TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reaction time of this method is long, the enzyme consumption is large, the conversion rate is low, and the product is a mixture of monoester and diester

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Add 100mg sucrose (0.292mmol) into a 25mL stoppered single-port reaction flask, dissolve it completely with 5mL mixed organic solvent tert-butanol-dimethyl sulfoxide (DMSO / tert-butanol=1 / 4, V / V), preheat After reaching 40°C, prepare four portions, add 500 mg each of Lipozyme TLIM, Novozym 435, Lipozyme TLI00, and Lipozyme RMIM to each portion, shake at 200 rpm for 30 min at the corresponding temperature of 40°C, and then add 300 μL of vinyl acetate (3 mmol) each, Place in a shaking incubator at 40° C., and react for 9 hours at a rotation speed of 200 rpm to obtain a crude sucrose ester solution. Samples were taken for HPLC analysis to detect the content of sucrose-6-acetate, and the corresponding molar conversion rates were calculated to be 77%, 37%, 15% and 6%, respectively.

Embodiment 2

[0018] According to the method of Example 1, wherein tert-butanol and DMSO in the mixed organic solvent are mixed in the ratio of 2: 1, 3: 1, 4: 1 and 5: 1 respectively, four parts are prepared, and 500 mg of Lipozyme is added in each part TLIM was reacted with 300 μL vinyl acetate for 12 hours, and the content of sucrose-6-acetate was detected by HPLC analysis, and the corresponding molar conversion rates were calculated to be 27%, 43%, 79% and 53%, respectively.

Embodiment 3

[0020] According to the method of Example 1, wherein 500 mg of Lipozyme TLIM is used as a catalyst, six parts are prepared, and 60, 120, 180, 240, 300, 360 μL of vinyl acetate are respectively added in each part, and the sucrose- The content of 6-acetate, the calculated corresponding molar conversions are 32%, 62%, 65%, 76%, 74% and 68%, respectively.

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PUM

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Abstract

The invention discloses a method for preparing sucrose-6-acetic ester by an enzymatic method, and belongs to the technical field of biocatalysis. In the method, in a non-aqueous medium tertiary butanol-dimethyl sulfoxide system, sucrose and vinyl acetate are catalyzed by lipase to perform a transesterification reaction so as to synthesize the sucrose-6-acetic ester. The method has the advantages of mild reaction condition, high reaction selectivity, single reaction product, high conversion rate, product safety and the like, and provides a novel feasible way for preparing the sucrose-6-acetic ester by a biological method.

Description

technical field [0001] The invention relates to a method for preparing sucrose-6-acetate by using lipase as a catalyst, belonging to the technical field of biocatalysis. Background technique [0002] Sucrose-6-acetate is a key intermediate in the synthesis of the sweetener sucralose. Sucralose (4,1',6'-trichloro-4,1',6'-trideoxygalactose) is a non-nutritive, low-energy sweetener obtained by chlorination of sucrose. About 600 times that of sucrose. Sucralose has good solubility and stability, does not participate in metabolism in the human body, will not cause blood sugar fluctuations, will not be converted into fat, and will not be used as a sugar source by dental caries bacteria. At present, it has been approved as a sweetener by more than 30 countries including China, and is widely used in the food field. [0003] In 1976, Professor Hough of the United Kingdom cooperated with Tate & Lyle to research and invent sucralose. In 1979, Tate & Lyte first applied for a patent f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12
Inventor 郑璞孙志浩王勍
Owner HANGZHOU XINFU TECH CO LTD
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