Preparation method of capillary probe array used for analyzing biochips

A biochip and probe array technology, applied in the field of capillary probe array preparation, can solve the problems of high processing cost, slow production speed, expensive precision instruments, etc., and achieve the goal of accelerating production speed, reducing processing cost, and reducing dependence Effect

Active Publication Date: 2010-11-17
成都艾迪恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods require high technical requirements, high processing costs, slow production speed, and require relatively expensive precision instruments.

Method used

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  • Preparation method of capillary probe array used for analyzing biochips
  • Preparation method of capillary probe array used for analyzing biochips
  • Preparation method of capillary probe array used for analyzing biochips

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] see figure 1 , the inlet of the capillary 1 is inserted into a small liquid storage test tube A, and the small liquid storage test tubes A are arranged at intervals and are respectively equipped with mineral oil 2 and probe solution 3 . The outlet of the capillary 1 is connected to a horizontal liquid storage tank 4 , and gravity is used to drive the liquid to flow, so that the carrier current 5 carries the droplet sequence 6 to flow in the capillary 1 . After the flow reaches the predetermined position, the flow is stopped and the immobilization reaction of the probe is carried out to complete the processing of the biochip.

[0020] Figure 4 It is the fluorescent signal intensity obtained by fixing nucleic acid probes of different concentrations for 30 minutes. The probe contains 20 bases, 3' labeled with FITC, 5' labeled with amino groups, through chemical reactions with free aldehyde groups modified on the capillary wall and is fixed in the capillary.

Embodiment 2

[0022] see figure 2 , the capillary 1 is a plurality of parallel capillaries, each channel has an inlet and an outlet, and a plurality of parallel probe arrays are processed for analysis of a plurality of parallel biochips. exist figure 2 , each tag is associated with figure 1 same.

Embodiment 3

[0024] see image 3 , the outlet of the capillary 1 is connected with a syringe, and the negative pressure generated by the syringe drives the flow of the liquid. exist image 3 , each tag is associated with figure 1 same.

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Abstract

The invention discloses a preparation method of a capillary probe array used for analyzing biochips, which relates to the biochips. The preparation method comprises the following steps of: introducing solution into a capillary tube; arranging liquid storage tanks or small test tubes stored with mineral oil or other organic solvents immiscible with water and probe solution at intervals for liquid supply; replacing feed liquid at an inlet of the capillary tube to realize a liquid droplet sequence of 'the mineral oil, the probe solution, the mineral oil, the probe solution, the mineral oil, ...'in the capillary tube, wherein the mineral oil serves as a carrying flow, and the probe solution serves as liquid droplets flowing in the carrying flow; when the probe liquid droplet sequence flows to a preset position in the capillary tube, stopping flowing the probe liquid droplet sequence to ensure that probes in the liquid droplets can be fixed to the tube wall of the capillary tube through reaction or adsorption spontaneously or under the external initiations of light, electricity, magnetism and the like; after finishing a fixation reaction, discharging the mineral oil or other organic solvents immiscible with water and the probe liquid droplets; and performing cleaning by using buffer solution to finish all the steps.

Description

technical field [0001] The invention relates to a biochip, in particular to a method for preparing a capillary probe array for biochip analysis. Background technique [0002] Biochips immobilize a large number of biomolecules or materials (such as nucleic acid fragments, proteins, drugs or receptors, cells or tissues, etc.) Carry out specific adsorption or reaction to realize the detection of sample information. Thousands of reactions are performed simultaneously on a single chip, with the capability of massively parallel analysis. Biochips are generally processed on carrier materials such as glass wafers, silicon wafers, and nylon membranes. The method of making probe arrays is mainly the spotting method (Schena M, Shalon D, Davis R W. et al. Science., 1995, 20: 467 -470) and in-situ synthesis (Fodor S P A, Read J L, Pirrung M C, et al.Science, 1991, 251: 767-773), the spotting method is to use a spotting instrument to spot the probe on the surface of the carrier and then...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/53G01N33/68
Inventor 陈宏瞿祥猛
Owner 成都艾迪恩生物科技有限公司
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