Method for enhancing fermentation level of Bifidobacterium by utilizing lycopene
A technology of bifidobacteria and lycopene is applied in the direction of adding compounds to stimulate growth, which can solve the problems of no patent documents and reports, and achieve the effect of cost saving
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Embodiment 1
[0026] (1) Dispersing lycopene with a content of 95% by weight into a uniform particle powder with a particle diameter of 1-3 mm.
[0027] (2) Take by weighing 10 grams of lycopene, 30 kg of glucose, 20 kg of peptone, 1 kg of dipotassium hydrogen phosphate, and 1 kg of salt, add it into a fermentation tank containing 1 ton of purified water, and adjust the pH to below 6.0 with phosphoric acid, after stirring and dissolving, After sterilizing at 121°C for 30 minutes, cool to 30°C.
[0028] (3) Inoculate 10 liters of the cultured bacterial solution, and control the temperature to 30° C. for anaerobic fermentation. When the pH naturally drops below 4.0, adjust the pH to about 4.5 with alkali. After 20 hours of fermentation, the reducing sugar decreased to 0.08%, and the bifidobacterium fermentation broth was obtained, and the count of viable bacteria in the fermentation broth was measured to be 31.2 billion / ml. Further, the fermented liquid was made into a solid bifidobacteria ...
Embodiment 2
[0030] (1) Dispersing lycopene with a content of 10wt% into a uniform particle powder with a particle diameter of 1-3 mm.
[0031] (2) Take by weighing 100 grams of lycopene, 40 kg of glucose, 30 kg of peptone, 1 kg of dipotassium hydrogen phosphate, and 1 kg of salt, add it into a fermentation tank containing 1 ton of purified water, and adjust the pH to below 6.0 with phosphoric acid, after stirring and dissolving, Sterilize at 121°C for 30 minutes and cool to 30°C.
[0032] (3) Inoculate 10 liters of the cultured bacterial solution, and control the temperature to 30° C. for anaerobic fermentation. When the pH naturally drops below 4.0, adjust the pH to about 4.5 with alkali. After 24 hours of fermentation, the reducing sugar decreased to 0.1%, and the bifidobacterium fermentation broth was obtained, and the count of viable bacteria in the fermentation broth was measured to be 40.5 billion / ml. Further, the fermented liquid was made into a solid bifidobacteria preparation b...
Embodiment 3
[0034] (1) Crush the tomato powder into a 20-mesh powder.
[0035] (2) Take by weighing 1kg of tomato powder powder, 30kg of glucose, 20kg of peptone, 1kg of dipotassium hydrogen phosphate, and 1kg of salt, add in a fermentation tank containing 1 ton of purified water, and adjust the pH to below 6.0 with phosphoric acid, after stirring and dissolving, 121 After sterilizing at ℃ for 30min, cool to 30℃.
[0036] (3) Inoculate 10 liters of the cultured bacterial solution, and control the temperature to 30° C. for anaerobic fermentation. When the pH naturally drops below 4.0, adjust the pH to about 4.5 with alkali. After 24 hours of fermentation, the reducing sugar decreased to 0.06%, and the bifidobacterium fermentation broth was obtained, and the count of viable bacteria in the fermentation broth was measured to be 35.2 billion / ml. Further, the fermented liquid was made into a solid bifidobacteria preparation by a conventional method, and the content of bifidobacteria was meas...
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