Method for preparing biological polysaccharide self-assembly modificatory chitosan antibacterial biological material

A technology of biological polysaccharides and biomaterials, applied in medical science, prostheses, etc., can solve the problems of antibacterial biomaterials that have not been reported, and achieve the effect of improving surface biological properties, simple preparation process, and easy control

Inactive Publication Date: 2013-06-12
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the preparation of antibacterial biomaterials by using lentinan and its derivatives to modify the surface of biomaterials.

Method used

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  • Method for preparing biological polysaccharide self-assembly modificatory chitosan antibacterial biological material
  • Method for preparing biological polysaccharide self-assembly modificatory chitosan antibacterial biological material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of biopolysaccharide self-assembly modified chitosan antibacterial biomaterial includes the following steps:

[0035] 1) Preparation of fungal polysaccharide derivative-lentinan sulfate: add 0.6 g of lentinan into a reaction flask containing 50 mL of dimethyl sulfoxide, stir at 20°C for 12 hours, and then slowly at a speed of 2 mL / min Add 9mL pyridine dropwise, continue stirring for 30 minutes, then place the reaction flask in an ice bath, and slowly add 3.7mL chlorosulfonic acid (of which chlorosulfonic acid) to a constant pressure funnel at a rate of 0.5mL / min under stirring. The molar ratio to pyridine is 1:2), then the temperature is raised to 80°C and the reaction is stirred for 100 minutes. After the reaction is stopped, it is cooled to room temperature and the concentration is 5wt% NaOH (a concentration of 5wt% means 100g NaOH solution contains 5g NaOH) solution Adjust the pH to 7.0 to obtain the reaction solution; then pour the obtained react...

Embodiment 2

[0042] The preparation method of biopolysaccharide self-assembly modified chitosan antibacterial biomaterial includes the following steps:

[0043] 1) Preparation of fungal polysaccharide derivative-lentinan sulfate: add 1.2 g of lentinan into a reaction flask containing 100 mL of dimethyl sulfoxide, stir at 30°C for 18 hours, and then slowly at a rate of 4 mL / min Add 18mL pyridine dropwise, continue stirring for 40 minutes, then place the reaction flask in an ice bath, use a constant pressure funnel to slowly drop 7.4mL chlorosulfonic acid (where chlorosulfonic acid and The molar ratio of pyridine is 1:2), then the temperature is raised to 80°C and the reaction is continued stirring for 120 minutes. After the reaction is stopped, it is cooled to room temperature, and the pH is adjusted to 7.0 with NaOH with a concentration of 5 wt% to obtain a reaction solution; and then the obtained reaction solution is injected In a regenerated cellulose dialysis bag (36mm, Mw: 8000-14000), di...

Embodiment 3

[0050] The preparation method of biopolysaccharide self-assembly modified chitosan antibacterial biomaterial includes the following steps:

[0051] 1) Preparation of fungal polysaccharide derivative-lentinan sulfate: add 0.6 g of lentinan into a reaction flask containing 50 mL of dimethyl sulfoxide, stir at 25°C for 16 hours, and then slowly at a speed of 3 mL / min Add 18mL pyridine dropwise, continue to stir for 35 minutes, then place the reaction flask in an ice bath, use a constant pressure funnel to slowly drop 3.7mL chlorosulfonic acid (where chlorosulfonic acid and The molar ratio of pyridine is 1:2), then the temperature is raised to 80°C and the reaction is continued stirring for 120 minutes. After the reaction is stopped, it is cooled to room temperature, and the pH is adjusted to 7.0 with NaOH with a concentration of 5 wt% to obtain a reaction solution; and then the obtained reaction solution is injected In the regenerated cellulose dialysis bag (36mm, Mw: 8000-14000), d...

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Abstract

The invention relates to a method for preparing a biological polysaccharide self-assembly modificatory chitosan antibacterial biological material, which is characterized by comprising the following steps: (1) preparing a lentinan derivant, i.e. lentinan sulfate; (2) preparing a chitosan membrane base material; (3) preparing a lentinan sulfate solution and a chitosan solution; and (4) carrying outlayer-by-layer self-assembly of the lentinan sulfate and the chitosan on the surface of the base material: respectively carrying out alternative self-assembly on the chitosan membrane base material in the lentinan sulfate solution and the chitosan solution for 5 times to obtain a surface layer-by-layer self-assembly modificatory chitosan mebrane containing 5 bilayers with the outermost layer of the lentinan sulfate, washing and drying to obtain the biological polysaccharide self-assembly modificatory chitosan antibacterial biological material. The chitosan antibacterial biological material prepared by the method has excellent antibacterial activity; and the method has simple preparation process, easy control, mild preparation conditions and low cost.

Description

Technical field [0001] The invention belongs to the technical fields of biological materials, polymer chemistry and molecular self-assembly, and particularly relates to a method for preparing a chitosan antibacterial biological material modified by self-assembly of biological polysaccharides. Background technique [0002] In recent years, the application of biomedical polymer materials in the medical field has become increasingly widespread, and more and more polymer materials have been used as substitute materials for human organs and tissues. However, when biomaterials are implanted inside the human body, they tend to trigger a series of body defense reactions such as body inflammation and blood coagulation. In addition, the infection of bacteria and other microorganisms during the implantation process can easily lead to problems such as postoperative inflammation, which greatly affects the application of biomedical polymer materials in the medical field. Therefore, it is requ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/54C08J7/00C08J7/12C08J5/18C08J3/24C08L5/08C08B37/00
Inventor 王艺峰洪群峰徐敏姚祥雨熊燕飞
Owner WUHAN UNIV OF TECH
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