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Growth factor-loaded collagen group composite material as well as preparation method and application thereof

A growth factor and composite material technology, applied in medical science, prosthesis, etc., can solve the problems of uncontrolled release of growth factors, loss of growth factor activity, waste of growth factors, etc., and achieve good biocompatibility and preparation process The effect of mild conditions and stable structure

Inactive Publication Date: 2013-04-10
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, severe conditions are used in most research processes, such as the use of organic solvents, which may cause the loss of activity of growth factors; at the same time, most of the release of growth factors has a burst release phenomenon, and the release direction of growth factors is not controlled. It can be released not only in the diseased tissue, but also around the diseased defect tissue, resulting in unforeseen consequences and a waste of the growth factor itself

Method used

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  • Growth factor-loaded collagen group composite material as well as preparation method and application thereof
  • Growth factor-loaded collagen group composite material as well as preparation method and application thereof
  • Growth factor-loaded collagen group composite material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of loose and dense collagen membranes

[0026] 1. Preparation of bovine tendon type Ⅰ collagen swelling solution

[0027] The fresh beef tendons purchased in the market were fully washed, the peritendon tissue, tendon sheath and fascia were removed, and after washing, they were frozen in a freezer. Take out the frozen beef tendon and cut it into slices about 1mm thick, add ficin solution (concentration 0.05-0.25%) for treatment, stir and put it in an incubator for enzymolysis for 24 hours, then add excess H 2 o 2 Terminate the reaction, wash with distilled water 3-4 times, add 0.3% malonic acid solution to swell for 24 hours after decanting. The swollen tendons were fully stirred for 6 hours, and the viscosity was adjusted with 0.3% malonic acid solution, filtered through a 80-120 mesh stainless steel mesh under positive pressure to remove impurities and unswollen substances, and packaged.

[0028] 2. Preparation of air-dried collagen membrane....

Embodiment 2

[0038] Embodiment 2: Preparation of chitosan-heparin-bFGF nanoparticles by ion cross-linking

[0039] 1. Chitosan (molecular weight 10000, degree of deacetylation 85%) was dissolved in 1% acetic acid solution, filtered and dialyzed for 4 days, freeze-dried to obtain purified chitosan.

[0040] 2. Weigh 0.2g chitosan and dissolve in 5ml 1% acetic acid, 0.1mol L -1 Adjust the pH to 6.0 with NaOH, set the volume to 100ml, and obtain a concentration of 2mg·ml -1 Chitosan solution is filtered to remove impurities therein.

[0041] 3. At 1mg·ml -1 Add the expected amount of bFGF to the heparin (molecular weight: 12,000) solution and mix overnight (8-12 hours). At 4°C, with stirring at 700rpm, 2ml of 2mg·ml -1 Chitosan solution drop by drop into 5ml 1mg·ml -1 In the composite bFGF heparin, the dropping speed is 20 drops / min, and the chitosan-heparin nanoparticles loaded with bFGF are obtained.

[0042] The prepared nanoparticles are relatively uniform in size observed under tr...

Embodiment 3

[0043] Example 3: Preparation of bFGF-loaded collagen-based composite material

[0044] Wet the cross-linked loose layer collagen membrane prepared in the above example with double distilled water and spread it flat, and slowly drop the Ch-He nanoparticles loaded with bFGF on the surface to cover the entire surface. Air-dry at 4°C until there is no mobile phase liquid on the surface of CFF, then wet the cross-linked dense layer collagen film and spread it on the surface of the cross-linked loose layer collagen film with a small amount of liquid remaining, and air-dry thoroughly at 4°C to obtain bFGF-loaded collagen Matrix composite material, wherein the amount of bFGF is 100ng / cm -2 .

[0045] Spray gold on the cross-section of the double-layer material, and it can be seen by field emission scanning electron microscopy ( image 3 ): The composite material is composed of two layers. The dense layer structure is dense and smooth, and the loose layer is loose and porous. This s...

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Abstract

The invention discloses a growth factor-loaded collagen group composite material and a preparation method thereof. The growth factor-loaded collagen group composite material is prepared from collagen, a growth factor, chitosan and sodium heparin, a dense layer collagen film and a loose layer collagen film form a double-layer structure, and a growth factor-loaded chitosan-heparin nano particle is compounded between the double layers. The dosage of the growth factor is 50-10,000ng / cm<2>; and the average thickness of the double-layer film is 1-4mm. The preparation method comprises the steps of preparing the dense collagen film and the loose collagen film, preparing the growth factor-loaded nano particle, compounding the collagen films and the nano particle, and the like. The collagen group composite material prepared by the invention has orient difference control slow release function on the loaded growth factor, has a slow release period longer than 20 days, maintains the activity of the growth factor and can be used for the restoration treatment of the disease defect tissue.

Description

Technical field: [0001] The invention relates to a collagen-based composite material loaded with growth factors and its manufacturing method and application. The material has a directional and differential controlled and slow-release effect on the growth factors loaded on it, and can be used for repairing and treating diseased and defective tissues. Background technique: [0002] Collagen is the main component of the extracellular matrix (ECM), which is a structural protein, accounting for about 1 / 3 of the total protein mass in mammals, and exists in large quantities in connective tissues or organs such as skin, ligaments, cartilage, and muscle bonds. Collagen has good biocompatibility, has no toxic side effects and irritation when implanted in the body, is not easy to cause the body's immune response, and is also conducive to cell adhesion, proliferation and differentiation, accelerates wound healing, and can be degraded to provide enough for new tissues. The space is widel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/44
Inventor 张其清王建华李学敏宿广昊史佳巍
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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