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Lactic acid production process with fermentation and expanded bed for in-situ adsorption coupling

A technology of expanding bed and lactic acid, applied in the biological field, can solve the problems of low separation efficiency, bed clogging, etc., achieve high separation efficiency, improve yield and conversion rate, and relieve feedback inhibition.

Inactive Publication Date: 2010-12-15
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fluidized bed adsorption can directly treat the feed containing solid particles, but the separation efficiency is low, and it has to be improved by circulating samples; although the separation efficiency of fixed bed chromatography is high, the solid particles in the feed often cause bed separation. The clogging must be preceded by a series of pretreatment processes before the chromatographic operation

Method used

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  • Lactic acid production process with fermentation and expanded bed for in-situ adsorption coupling
  • Lactic acid production process with fermentation and expanded bed for in-situ adsorption coupling
  • Lactic acid production process with fermentation and expanded bed for in-situ adsorption coupling

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Effect test

Embodiment 1

[0042] The device of the present invention includes: a high-level feeding tank, a fermentation tank, a transmission pump, a four-way valve, an expanded bed chromatography column, an eluent storage tank, a regeneration liquid storage tank, a stop valve, and a lactic acid storage tank. as attached figure 1 shown.

[0043] The device can have two or more expanded bed chromatography columns as required, the top of which is connected to the lactic acid storage tank and the circulating fluid inlet of the fermentation tank through a four-way valve; the bottom is connected to the eluent storage tank, The regeneration liquid storage tank is connected to the outlet of the circulating liquid of the fermenter;

[0044] The fermenter is equipped with a stirring device, a feeding tank and an electrode, and the bottom is respectively provided with an inlet, an air inlet and a material outlet for the fermentation circulation liquid.

[0045] The present invention is a process of utilizing e...

Embodiment 2

[0051] see figure 1 . The lactic acid production device coupled with fermentation and expanded bed in-situ adsorption of the present invention includes a fermentation tank, two parallel expanded bed chromatography columns, two transfer pumps, 4 shut-off valves, 2 four-way valves, and a high-level feeding tank , eluent storage tank, regeneration solution storage tank and lactic acid storage tank.

[0052] The fermenter 2 is connected to the No. 1 expanded bed chromatography column 5 and the No. 2 expanded bed chromatography column 6 through pipelines, and the bottom openings of the No. 1 expanded bed chromatography column 5 and the No. 2 expanded bed chromatography column 6 are respectively connected with cut-off Valves 7 and 8 are connected to the bottom outlet of fermenter 2 and eluent storage tank 12 and regeneration liquid storage tank 14 through No. 2 four-way valve 9; No. 1 expanded bed chromatography column 5 and No. 2 expanded bed The top opening of the chromatography...

Embodiment 3

[0066] The expanded bed system described above was used. Expanded bed chromatography column coupled in situ separation batch fermentation. Depending on the initial sugar content in the culture medium, the fermentation ended within 48 hours. The results are shown in Table 2.

[0067] Table 2 In situ separation batch fermentation results

[0068]

[0069] Example 3

[0070] The types of resins used for expanded bed chromatography packed columns are shown in Table 3. The resins were first soaked in deionized water for 24 hours, and then pretreated three times with "1mol / L HCl, water, 1mol / L NaOH, water". Treat it as -OH type, and finally wash the bed with sterile water to neutral, that is, the preparation is complete. After adsorption saturation, use 10wt%-40wt% H 2 SO 4 Eluted as a desorbent. Then regenerate with 1mol / L NaOH or KOH. After one cycle of "adsorption, elution, and regeneration", the adsorption performance and resolution rate of the resin are shown in Table ...

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Abstract

The invention discloses a lactic acid production device for fermentation and separation coupling and a lactic acid fermentation process using expanded bed in-situ adsorption to realize online separation. In the invention, an expanded bed and a fermentation tank are coupled, the online separation of lactic acid fermentation is realized by using the expanded bed method, so that the feedback inhibition of lactic acid is relieved, the yield and the conversion rate of the lactic acid fermentation are increased, and meanwhile, the problem of bed blockage by thalli when a fixed bed is used for absorbing the lactic acid in the prior art. Untreated fermentation liquor is permitted to directly enter a chromatographic column. Because the synchronization of fermentation and separation is realized, the cost of downstream extraction and separation in the prior art is greatly reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology and discloses a new process for producing lactic acid. Specifically, the fermentation method is used to produce lactic acid; during the fermentation process, the process of separating lactic acid by expanding the bed is coupled with the fermentation process to realize the on-line separation of lactic acid. Background technique [0002] Lactic acid is an important multipurpose organic acid, widely used in food, medicine, chemical industry, tanning, textile, environmental protection and agricultural production and other fields. The derivative of lactic acid - polylactic acid (PLA), can be used to produce biodegradable fibers, biodegradable plastics and biodegradable medical materials, etc., which has attracted the attention of more and more scholars. [0003] Lactic acid production can be divided into chemical synthesis and microbial fermentation. Among them, the chemical synthesis method has serious ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12P7/56B01D15/08B01D15/36C12R1/225
CPCC12M47/12C12M47/10C12M41/12C12M29/04
Inventor 鲁明波余龙江曾翔何峰何谧
Owner HUAZHONG UNIV OF SCI & TECH
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