Novel cervical exfoliated cell separation method

Abstract

The invention discloses a novel cervical exfoliated cell separation method, which is characterized by: placing cervical exfoliated cells in phosphate buffer solution (PBS) and uniformly mixing by whirling to obtain cell suspension; adding the cell suspension into mucilage separating solution; centrifuging, removing supernate, adding the phosphate buffer solution (PBS) again and re-suspending sediment to obtain re-suspended cell suspension; and adding the re-suspended cell suspension into neutral granular cell separating solution Ficoll, centrifuging, removing supernate and obtaining the settlement which is pure cells after centrifugation. The method of the invention greatly purify clinically extracted cervical exfoliated cell specimens, adopts simple and convenient operation steps, is suitable for large-scale cell separation with stable and reliable effect, can obtain high-purity target cells, has high actual use value and a promising market prospect.

Description

technical field [0001] The invention relates to a method for separating cervical exfoliated cells. Background technique [0002] Cervical cancer is one of the common malignant tumors in gynecology. Its incidence rate is second only to breast cancer, ranking second in female malignant tumors, and its mortality rate ranks first in gynecological malignant tumors. In recent years, cervical cancer patients have been increasing at an annual rate of 2% to 4%. At the same time, compared with developed countries, the incidence of cervical cancer in developing countries is 6 times that of developed countries due to imperfect cervical screening. , and 80% of the patients were diagnosed with invasive cancer, and cervical cancer has a long period of reversible precancerous lesions, so early detection of precancerous lesions is the key to prevent and treat cervical cancer. [0003] At present, the main methods for screening cervical cancer include cervical smear exfoliation cytology exam...

AI Technical Summary

Problems solved by technology

[0003] At present, the main methods for screening cervical cancer include cervical smear exfoliation cytology examination, cervical epithelial liquid-based cytology examination, etc., but there are certain limitations. Precancerous lesions are symptoms of a gradual transition from benign to malignant, including: 1. The number of keratinocytes in cervical smears continues to increase, and the cells in the smears are continuously affected by estrogen, and the degree of keratinization is equivalent to the cycle The highest level before mid-ovulation; 2. Atypical or precocious keratinocytes; 3. Huge cells, or small cells; 4. Abnormally large nuclei, active and immature, densely stained nuclear chromatin, often in the cytoplasm Granules; 5. Nuclei lobulated and multinucleated with various layers of squamous epithelial cells; 6. Proliferation of underlying cells, often with halo around the nucleus; 7. Severe hyperplasia of reserve cells

[0004] In the currently widely used cervical epithelial liquid-based cytology examination in clinical practice, the cells undergo only one separation process and then directly enter the production step, although the mucus, blood cells, inflammation However, some mucus, especially inflammatory neutrophils, can still be seen under the microscope after the film is made, which affects the detection rate of abnormal cells. Clinically provide more accurate auxiliary diagnostic information

Images