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Colloidal gold colloidal gold detection test paper strip of shrimp TSV (Taura Syndrome Virus)

A technology for detecting test strips and colloidal gold, which is applied to viral peptides, antiviral immunoglobulins, and measuring devices. It can solve the problems of equipment limitations and high costs, and achieve the effects of maintaining activity, low cost, and reducing use costs.

Inactive Publication Date: 2013-05-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA method is relatively mature, but prone to non-specificity; electron microscope observation method is intuitive, but expensive; RT-PCR method is sensitive and fast, but expensive and has equipment limitations
So far, there is no report on the establishment of TSV immune colloidal gold test strip method

Method used

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  • Colloidal gold colloidal gold detection test paper strip of shrimp TSV (Taura Syndrome Virus)
  • Colloidal gold colloidal gold detection test paper strip of shrimp TSV (Taura Syndrome Virus)
  • Colloidal gold colloidal gold detection test paper strip of shrimp TSV (Taura Syndrome Virus)

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Embodiment Construction

[0042] The steps of the concrete technical route that the present invention adopts:

[0043] 1. Preparation and purification of TSV recombinant protein pET32a-VP1cr

[0044] 1 Preparation of TSV recombinant protein pET32a-VP1cr

[0045] Amplified the VP1 conserved region gene VP1cr (amplification position 7949bp-8789bp, the whole genome sequence has been released accession number: DQ104696, Huang Xinxin, Lu Chengping. Whole gene sequencing and molecular structure prediction of Taura syndrome virus Chinese strain ZHZC3[J].China Virology, 2006, 21(3): 267-272), constructed the high-efficiency expression vector pET32a-VP1cr, transformed it into B121 strain, and induced the expression of recombinant protein with IPTG.

[0046] 2 Purification of TSV recombinant protein pET32a-VP1cr

[0047] After confirming that the recombinant protein is mainly expressed in the form of inclusion bodies, the inclusion bodies were washed once with inclusion body washing solution, and centrifuged a...

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Abstract

The invention relates to a colloidal gold colloidal gold detection test paper strip of a shrimp TSV (Taura Syndrome Virus), belonging to the technical field of colloidal gold immunoassay. A colloidal gold-rabbit anti-mouse iga pET32a-VP1cr polyclonal antibody bonder glass fiber membrane is arranged at one end of a test paper strip soleplate, an antibody solid-phase nitrocellulose membrane is arranged in the middle and scribed with an anti-mouse iga pET32a-VP1cr polyclonal antibody detection line (T line) and a goat anti-rabbit IgG control line (C line), and absorbent paper is arranged at the other end of the soleplate. When the TSV is carried in a detected sample, the TSV is firstly combined with the colloidal gold-rabbit anti-mouse iga pET32a-VP1cr polyclonal antibody into a compound andcaptured when moving forwards under the capillarity to the anti-mouse iga pET32a-VP1cr polyclonal antibody, i.e. double-antibody sandwiched specific binding occurs, colloidal gold particles at the detection line are enriched to be red so as to detect whether the sample contains the TSV or not. The method has the advantages of rapidness, accuracy, convenience, and the like, is particularly suitable for on-site detection and primary screen detection and has wide prospects.

Description

technical field [0001] The invention relates to a colloidal gold detection test strip for prawn taura syndrome virus (TSV), and belongs to the technical field of colloidal gold immune detection. Background technique [0002] Shrimp Taura Syndrome (TS) is a disease caused by Taura Syndrome Virus (TSV) infection. P. stylirostris) outbreaks and high mortality rates. The cumulative mortality rate of Litopenaeus vannamei infected by it is 40%-100%. The disease was discovered by Ecuadorian researchers in a Litopenaeus vannamei farm near the mouth of the Taura River in 1992, and the new disease was named after the Taura River area, which must be declared by the World Organization for Animal Health (OIE) animal disease. [0003] At present, the available methods for diagnosing TS and its pathogen TSV include: traditional methods, immunological methods and molecular biological methods. Traditional methods such as: clinical symptoms, histopathology, animal experiments. Immunologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/531C12N15/40C12N15/63C07K14/08C07K16/10
Inventor 费荣梅柏琴琴陆承平赵彦华
Owner NANJING AGRICULTURAL UNIVERSITY
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