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Pseudomonas citronelloalis strain capable of degrading estrogen and application thereof

A technology of Pseudomonas and estrogen, which is applied in the field of Pseudomonas citronellolii and its degradation of estrogen, can solve the problems of not being degraded and not being able to effectively remove estrogen, and achieve the effect of increasing the rate

Inactive Publication Date: 2010-12-22
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

Conventional active sewage treatment systems cannot effectively remove estrogen in raw water. Although estrone and 17β-estradiol can be partially degraded, 17α-ethinyl estradiol is basically not degraded, resulting in ng / L Levels of estrogen were detected (Ternes et al., Sci.Total.Environ.Vol.225, 91-99, 1999)

Method used

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  • Pseudomonas citronelloalis strain capable of degrading estrogen and application thereof
  • Pseudomonas citronelloalis strain capable of degrading estrogen and application thereof
  • Pseudomonas citronelloalis strain capable of degrading estrogen and application thereof

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Embodiment Construction

[0018] 1. Isolation, screening and preservation of the Pseudomonas citronellolis SS-2 bacterial strain provided by the present invention

[0019] The activated sludge in the denitrification and phosphorus removal process system of the sewage treatment plant was collected as a microbial source, and transported back to the laboratory under refrigeration. Prepare an inorganic medium containing 50mg / L estrogen as the sole carbon source, and inoculate it in a 500mL Erlenmeyer flask with 100mL of the above-mentioned inorganic medium according to the inoculum amount of 2.5% activated sludge. The inorganic component contained in the medium is K 2 HPO 4 , 0.1g / L; (NH 4 ) 2 HPO 4 , 0.1g / L; MgSO 4 ·7H 2 O, 0.02g / L; FeCl 3 , 0.01g / L; CaCl 2 2H 2 O, 0.1g / L; NaCl, 0.1g / L. Use 2‰ acetic acid to adjust the pH value of the medium to 7.0-7.2, and sterilize it in an autoclave at 121°C for 20 minutes. After the temperature drops to about 30°C, inoculate it under the ultra-clean bench. ...

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Abstract

The invention relates to a pseudomonas citronelloalis strain and application thereof in degrading estrogen, wherein the pseudomonas citronelloalis strain has the preservation number of CGMCC No.3634, can grow by using natural or synthetic estrogen as a unique carbon source, can efficiently degrade theelin (E1), 17beta-estradiol (E2) and 17alpha-ethinyl estradiol (EE2) and can be used for a micro-biological degradation technology for removing the estrogen. Under the culture condition of using the estrogen as the unique carbon source, the theelin and the 17beta-estradiol with the initial concentration of 2mg / L can be respectively degraded by 99 percent by the SS-2 strain within 36 hours, the 17alpha-ethinyl estradiol with the initial concentration of 4mg / L can be degraded by 93.6 percent within 168 hours, and estriol has no remarkable degradation phenomenon. The theelin is generated in the process of degrading the 17beta-estradiol, and the theelin is also gradually degraded. Because the pseudomonas citronellolis SS-2 strain has the characteristics of effectively degrading the natural and synthetic estrogen in an environment, the pseudomonas citronellolis SS-2 strain can be applied to an estrogen-contained waste sewage treatment technology or a soil remediation technology.

Description

technical field [0001] The invention relates to a strain of Pseudomonas citronellolis and its application in degrading estrogen, in particular to using the Pseudomonas citronellolis to effectively degrade natural estrogen estrone and 17β-estradiol Alcohol and the synthetic estrogen 17α-ethinyl estradiol. Background technique [0002] Estrone (Estrone, E1), 17β-Estradiol (17β-Estradiol, E2), and Estriol (E3) are natural estrogens, which are secreted by the ovaries of humans and animals, and will enter with feces and urine. Sewage treatment plants, soil, farmland and other environments. The synthetic estrogen 17α-ethinylestradiol (17α-Ethynylestradiol, EE2) is the main component of low-dose oral contraceptives, drugs for treatment of irregular menstruation and menopausal drugs, and will also be excreted into sewage treatment plants with feces and urine. figure 1 is the chemical structural formula of the above estrogen. The estrogen emitted by the livestock breeding industry...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38
Inventor 史江红宿凌燕韩蕊曹金玲
Owner BEIJING NORMAL UNIVERSITY
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