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Method for preparing ginsenoside Rg3 from alternaria alternata YS-2 and fermented ginseng stem-leaf total saponin thereof

A technology of stems and leaves of Alternaria genus and ginseng, which is applied in the field of microbiology and medicine to achieve the effects of low production cost, high product yield and simple nutritional requirements

Inactive Publication Date: 2010-12-29
YANBIAN UNIV
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Problems solved by technology

There are no reports in the literature at home and abroad on the research involving the fermentation and transformation of total saponins from ginseng stems and leaves by Alternaria bacteria

Method used

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  • Method for preparing ginsenoside Rg3 from alternaria alternata YS-2 and fermented ginseng stem-leaf total saponin thereof
  • Method for preparing ginsenoside Rg3 from alternaria alternata YS-2 and fermented ginseng stem-leaf total saponin thereof
  • Method for preparing ginsenoside Rg3 from alternaria alternata YS-2 and fermented ginseng stem-leaf total saponin thereof

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Embodiment Construction

[0023] In the method for preparing Rg3 by fermenting Alternaria alternata YS-2 used in the examples of the present invention to transform total saponins from ginseng stems and leaves, the fermentation method is: inoculate, cultivate and feed in a 250mL Erlenmeyer flask, and the fermentation temperature Adjust to 10-50°C, pH=2-8, shaker rotation speed 10-200r / min, fermentation time 5h-120h. The product was qualitatively and quantitatively detected by HPLC-MS, and Rg3 monomer was obtained by column chromatography.

[0024] The specific implementation is as follows:

[0025] Isolation of bacterial strains: Weigh 1g of soil from the roots of ancient cypress trees more than 600 years old collected from Qianshan District, Anshan City, Liaoning Province, and add it to a 99mL sterile saline conical flask equipped with a stirring bar in a biological safety cabinet. Place it on a magnetic stirrer and stir for 10-20min, so that the bacteria, spores or spores in the soil sample are evenl...

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Abstract

The invention discloses a method for preparing ginsenoside Rg3 from alternaria alternata YS-2 and fermented ginseng stem-leaf total saponin thereof. The alternaria alternata YS-2 is an alternaria alternata YS-2 CGMCC No.3353 strain; and during fermentation, the feeding amount of the ginseng stem-leaf total saponin is 1 to 10g / L, the fermentation temperature is 10 to 50 DEG C, the pH is 2 to 8, the revolution number of a shaker is 10 to 200r / min, and the fermentation time is 5 to 120 hours. The method provides the alternaria alternata YS-2 strain with stable trait, simple nutrition requirement and good growth for scale fermentation conversion of various ginsenosides and preparation of Rg3; and the method for preparing the Rg3 by fermenting the ginseng stem-leaf total saponin through the strain is simple and convenient, and has low production cost and high product yield.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and medicine, and relates to an Alternaria bacterium and its application, specifically refers to a kind of Alternaria bacterium fermenting and transforming the total saponins of ginseng stems and leaves, and preparing rare 20(S)-ginsenoside Rg3( 20 (S)-Ginsenoside Rg3, hereinafter referred to as Rg3) method. technical background [0002] Ginsenoside Rg3 is a tetracyclic triterpene saponin present in the natural medicine Panax ginseng with the molecular formula C 42 h 72 o 13 The relative molecular weight is 784. Ginsenoside Rg3 has the effect of inhibiting tumor growth, mainly acts on the G2 / M phase of the cell proliferation cycle, induces tumor cell apoptosis, selectively inhibits tumor cell adhesion and invasion, resists tumor metastasis, inhibits tumor angiogenesis, and regulates the body's immune function etc. Clinical experiments have proved that the inhibition rate of Rg3 on vari...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P33/00C12R1/645
Inventor 尹成日藏韫霞
Owner YANBIAN UNIV
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