Marine microalgae chloroplast expression vector and application thereof

Abstract

The invention discloses a marine microalgae chloroplast expression vector and application thereof. The marine microalgae chloroplast expression vector contains a homologous recombinant sequence I, a target gene expression case and a homologous recombinant sequence II, wherein the target gene expression case is positioned between the homologous recombinant sequence I and the homologous recombinant sequence II; 1st to 972nd bp of the homologous recombinant sequence I are 3' part of rns, and 973rd to 1,097th bp are 5' part of trnI; and 1st to 196th bp of the homologous recombinant sequence II are 3' part of trnA, and 197th to 1,483rd bp are 5' part of rnl. The homologous recombinant sequence ensures that the expression vector can be induced into the marine microalgae chloroplast genome by an electric shock method, which is advantageous to the way of inducing the vector into the chloroplast genome, greatly reduces conversion cost, and provides technical support for realizing that marine microalgae chloroplast is used as a bioreactor to produce high value-added products.

Description

technical field [0001] The invention relates to the field of microalgae genetic engineering, in particular to a marine microalgae chloroplast expression vector and application thereof. Background technique [0002] Since the first transgenic plant tobacco came out in 1984, the transformation of exogenous genes into the plant nucleus has been the main research direction of plant genetic engineering. However, due to the low expression level of exogenous genes in the plant nucleus, the unstable expression in offspring, and the ecological security caused by the easy diffusion of nuclear genes with pollen, there are other problems. Compared with traditional nuclear transgenesis, chloroplast transformation has many advantages: there are a large number of chloroplast copies in each plant cell, which makes the overexpression of foreign genes difficult to achieve in nuclear gene transformation; foreign genes are targeted by homologous recombination After integration in the chloropla...

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Problems solved by technology

[0004] Chlamydomonas reinhardtii is the only eukaryotic algae that has successfully transformed chloroplasts. In this transformation system, the promoter and terminator derived from chloroplasts are used to regulate the expression of foreign genes, and the vector is introduced into the chloroplast genome by gene gun technology, which has achieved success. However, the chloroplast transformation system of marine microalgae has not yet been established, and the introduction of traditional chloroplast transformation vectors into the recipient chloroplast genome is accomplished by gene gun technology, which is complex and costly

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