Lensless fluorescence imaging detection device and application
A fluorescence imaging and detection device technology, which is applied in the direction of fluorescence/phosphorescence, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of increasing the volume of detection devices, and achieve reduced volume and complexity, high sensitivity, and reduced The effect of restrictions on the use of small instruments
Active Publication Date: 2011-01-19
ZHEJIANG UNIV
View PDF4 Cites 12 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
In order to improve the signal quality, complex lens groups and precise position adjustment systems are often used, which not only increases the volume of the detection device, but also increases the requirements for user operations
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0026] see figure 1 , The device of the present invention is composed of a left excitation light source (1), a heating plate (2), a microchip (3), an optical filter (4), a CCD chip (5) and a right excitation light source (8).
[0027] see figure 2 , the microchip (3) is composed of upper and lower layers, the thickness of the lower layer of the microchip (3) is about 100 microns, to reduce the distance between the luminescent material and the CCD chip (5), the upper layer of the microchip (3) contains channels and The detection unit, when detecting, the upper layer of the microchip (3) is in close contact with the optical filter (4); the optical filter (4) is placed directly below the photosensitive area of the CCD chip (5); it is heated by the heating plate (2). The excitation light source (1) is irradiated in parallel to the side of the microchip; the collected fluorescent signal is transmitted to the computer through the CCD chip (5) for analysis and processing. The sa...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| thickness | aaaaa | aaaaa |
Login to View More
Abstract
The invention provides a lensless fluorescence imaging detection device. The device consists of left and right excitation light sources, a heating plate, a microchip, an optical filter, a CCD chip and a peripheral driving circuit of the device. Compared with the common microchip detector, the lensless fluorescence imaging detection device of the invention makes full use of the characteristics of small pixel and high integration level of the CCD chip, so a corresponding relationship between a detection unit in the microchip and each pixel of the CCD chip is established by shortening the distance between the CCD chip and a reactive material, and the volume and the complexity of a detection device are greatly reduced by directly imaging without a lens. Compared with the common micro fluorescence detection device, the lensless fluorescence imaging detection device has the advantages of simple structure and operation, low requirements on a detection chip, low cost, capacity of reducing the cost and loss of the microchip, application to thermal cycling PCR reaction or isothermal nucleic acid amplification, reasonable design, simple preparation and easy operation.
Description
technical field [0001] The invention relates to a fluorescence detection device of a microchip, in particular to a device capable of high-throughput detection of the fluorescence signal of a microchip without a lens. Background technique [0002] Due to the advantages of high integration and low reagent loss, microchips are widely used in many fields such as medical diagnosis, environmental protection, and drug screening. Fluorescence detection is an important method for detecting the reaction results of microchips. The basic principle is: the excitation light source is irradiated into the micropipe or microreaction chamber, the fluorescent substance obtains energy and emits fluorescence, which is converted into an electrical signal by a photoelectric conversion device and processed by a computer. [0003] Commonly used fluorescence detection devices need to filter and focus the excitation light and the excited fluorescence, so they include the following optical components: ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12Q1/68
Inventor 金伟牟颖周超金钦汉
Owner ZHEJIANG UNIV