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Method for extracting chicken egg yolk antibodies against infectious bursal disease

A chicken egg yolk antibody and bursal disease technology, applied in the direction of anti-viral immunoglobulin, egg-derived immunoglobulin, etc., can solve the problems of antibody loss, affecting the productivity of egg yolk antibody, etc., so as to reduce losses and facilitate production, promotion and application. , the effect of improving productivity

Inactive Publication Date: 2011-01-26
ZHENGZHOU HOUYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation and production process of yolk antibody is mature in the current production. The centrifugal precipitation method is mainly used to extract the antibody, which is more efficient, but in the case of high-speed centrifugation, it will cause the loss of antibody, thus affecting the production rate of yolk antibody.

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  • Method for extracting chicken egg yolk antibodies against infectious bursal disease

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Effect test

Embodiment 1

[0019] The method for extracting the yolk antibody of chicken infectious bursal disease of the present embodiment, its steps are as follows:

[0020] 1) Wash the immunized eggs, then wash them with sterile saline, dry them, then soak them in disinfectant at 42°C for 20 minutes, dry them, spray them with 95% alcohol for disinfection, and dry them for later use;

[0021] 2) Use an egg yolk separator to separate the egg yolk from the immunized eggs, break it up, add sterile saline equal to the volume of the egg yolk, then add penicillin and streptomycin at an amount of 2000U per ml of egg yolk, and then beat crushed and mixed to obtain egg yolk liquid;

[0022] 3) Add acetic acid-sodium acetate buffer solution with a pH of 4.8 to the egg yolk liquid, the amount added is 5 times the volume of the egg yolk liquid, mix well, let stand at 4°C for 10 hours, and take the supernatant;

[0023] 4) Add caprylic acid of 0.8% volume of the supernatant to the supernatant obtained in step 3)...

Embodiment 2

[0027] The method for extracting the yolk antibody of chicken infectious bursal disease of the present embodiment, its steps are as follows:

[0028] 1) Wash the immunized eggs, then wash them with sterile saline, dry them, then soak them in disinfectant at 42°C for 20 minutes, dry them, spray them with 95% alcohol for disinfection, and dry them for later use;

[0029] 2) Use egg yolk separator to separate, obtain egg yolk from immunized eggs, break it up, add sterile physiological saline equal to the volume of egg yolk, then add penicillin and streptomycin at an amount of 3000U per ml of egg yolk, and then break it up Mix well to obtain egg yolk liquid;

[0030] 3) Add acetic acid-sodium acetate buffer solution with a pH of 5.0 to the egg yolk liquid, the amount added is 6 times the volume of the egg yolk liquid, mix well, let stand at 5°C for 11 hours, and take the supernatant;

[0031] 4) Add octanoic acid at a volume of 0.9% of the supernatant to the supernatant obtained ...

Embodiment 3

[0035] The method for extracting the yolk antibody of chicken infectious bursal disease of the present embodiment, its steps are as follows:

[0036] 1) Wash the immunized eggs, then wash them with sterile saline, dry them, then soak them in disinfectant at 42°C for 20 minutes, dry them, spray them with 95% alcohol for disinfection, and dry them for later use;

[0037] 2) Use an egg yolk separator to separate the egg yolk from the immunized eggs, break it up, add sterile saline equal to the volume of the egg yolk, then add penicillin and streptomycin at an amount of 4000U per ml of egg yolk, and then break it up Mix well to obtain egg yolk liquid;

[0038] 3) Add acetic acid-sodium acetate buffer solution with a pH of 5.2 to the egg yolk liquid, the amount added is 7 times the volume of the egg yolk liquid, mix well, let stand at 6°C for 12 hours, and take the supernatant;

[0039] 4) Add caprylic acid of 1% volume of the supernatant to the supernatant obtained in step 3), mi...

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Abstract

The invention discloses a method for extracting chicken egg yolk antibodies against infectious bursal disease, which comprises the following steps of: taking an immune egg yolk, breaking the immune egg yolk, and purifying by respectively adopting acetic acid-sodium acetate buffer solution, octylic acid solution and ammonium sulfate; and filtering by using a filter membrane to obtain the egg yolk antibodies. In the method for extracting the chicken egg yolk antibodies against the infectious bursal disease, the acetic acid-sodium acetate buffer solution, the octylic acid solution and the ammonium sulfate are respectively adopted for purification, and the filter membrane is adopted for filtration finally, so the loss of the antibodies and the damage to the antibodies are reduced, and the productivity of the egg yolk antibodies is improved; and the method has the advantages of simple operation, and convenient production, promotion and application.

Description

technical field [0001] The invention relates to a preparation method of chicken yolk antibody for chicken infectious bursal disease, belonging to the technical field of disease immunity. Background technique [0002] Infectious bursal disease of chickens is a common disease in the poultry industry, which is prone to large-scale outbreaks and causes serious economic losses. At present, the main prevention methods include antibiotics, Chinese and Western medicines and their preparations, egg yolk antibodies and their biological agents, etc., among which the egg yolk antibodies are immunized with chicken infectious bursal disease antigens in healthy chickens, and the chicken infectious method is extracted from the yolks of immunized eggs. Antibody for bursal disease, this kind of yolk antibody after chicken immunization has a quick effect on chicken infectious bursal disease, significant curative effect, and strong pertinence, and has a good preventive effect on chicken infecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02C07K1/34C07K1/14
Inventor 吴红云郭俊清李建正
Owner ZHENGZHOU HOUYI PHARMA
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