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Echinococcosis granulosa gene engineering vaccine candidate P-29

A genetically engineered vaccine, the technology of Echinococcus granulosus, which is applied in the field of genetically engineered vaccines against hepatic hydatid disease, can solve the problems of toxic and side effects, drug resistance of chemotherapy patients, and intolerance of patients.

Inactive Publication Date: 2011-02-02
赵巍 +2
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Albendazole or mebendazole and other drugs can be used for chemotherapy in cases of early echinococcus minor or those with contraindications to surgery. Clinically, it has been found that these drugs have serious side effects, and some patients cannot tolerate them. At the same time, some chemotherapy patients may develop drug resistance

Method used

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  • Echinococcosis granulosa gene engineering vaccine candidate P-29
  • Echinococcosis granulosa gene engineering vaccine candidate P-29
  • Echinococcosis granulosa gene engineering vaccine candidate P-29

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Embodiment Construction

[0009] Preparation of Echinococcus granulosus Vaccine Candidate Molecules

[0010] 1. Use bioinformatics technology to search for target gene fragments

[0011] First log in to the GenBank (National Institutes of Health) database (http: / / www.ncbi.nlm.nih.gov / GeneBank / ), enter the species name - Echinococcus granulosus (Echinococcus granulosus), and query through Entrez Nucleotide The published Echinococcus granulosus gene sequence was searched, and the P-29 gene sequence was selected as a candidate gene for genetic engineering vaccine.

[0012] 2. Synthetic primers

[0013] Primers were designed according to the base sequence of P-29, and the primer sequences are listed in Sequence Tables 2 to 3 respectively.

[0014] See sequence 2 for the primer at the 5' end of the P-29 gene; see sequence 3 for the primer at the 3' end of the P-29 gene.

[0015] 3. Extraction of total RNA from Echinococcus granulosus

[0016] Take 200 mg of protoscoleia fresh or frozen in liquid nitroge...

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Abstract

The invention provides an echinococcosis granulosa gene engineering vaccine. The sequence of the diagnostic antigen P-29 gene of the echinococcosis granulosa Uruguay strain is retrieved from the Genbank of www.ncbi.nlm.nih.Gov, the P-29 gene is amplified through RT-PCR, with the total RNA of the echinococcosis granulosa pathogens of echinococcosis patients in Ningxia region, China as the template, and the DNA sequence of the amplified gene is consistent with that of the gene of the Uruguay strain. The cloned P-29 gene and the expression vector pET-28a are recombined through the recombinant DNA technology to transform and construct the engineered escherichia coli strain. The P-29 recombinant protein is prepared and the obtained P-29 recombinant protein immune mice are attacked by the protoscolex pathogens of echinococcosis patients in Ningxia region. Through computation in the experiments, the protective immunity of EgP-29 is 96.6%. The P-29 recombinant protein is proved to have immunology effect of the vaccines.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a genetic engineering vaccine for zoonotic parasitic diseases, in particular to a genetic engineering vaccine for hepatic echinococcosis. Background technique [0002] Echinococcus granulosus, commonly known as echinococcosis, is a parasitic disease caused by Echinococcus granulosus infecting humans and herbivorous livestock. Because Echinococcus granulosus mainly parasitizes in the human liver, the disease is also called hepatic echinococcosis. It is distributed worldwide, seriously endangering human health and the development of agricultural and animal husbandry economy, and is a global public health problem. According to the census in recent years, 25 provinces, municipalities and autonomous regions in my country have reported cases of the disease, among which seven provinces (districts) of Xinjiang, Ningxia, Gansu, Qinghai, Tibet, Sichuan and Inner Mongolia are the most prevalent,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P33/10A61K39/00
Inventor 赵巍师志云棘怀庆
Owner 赵巍
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