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A plant protein and mineral technology, applied in the field of fermented plant protein preparation, can solve the problem that cellulose cannot be effectively removed, and achieve low cost, environmental protection and good effect
Inactive Publication Date: 2013-07-10
钱国英 +3
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However, in this method, the feed water is sterilized water; non-starch polysaccharides such as cellulose in the fermented soybean meal cannot be effectively removed, and this fermentation process is only applicable to the plant protein source of soybean meal, and has no effect on other vegetable proteins. effect
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Embodiment 1
[0021] The present embodiment selects rapeseed meal as raw material.
[0022] (1) Crush the rapeseed meal, pass through a 40-mesh sieve, mix well, weigh 50g of rapeseed meal powder and put it into CO 2 Gas purged 500ml anaerobic fermentation bottle.
[0023] (2) When slaughtering ruminants such as cattle, sheep, deer, etc., quickly separate the rumen without opening the rumen, turn the rumen upside down several times, and mix the rumen contents evenly. Then cut the rumen with scissors, the opening of the rumen should not be too large, and it is inconvenient to collect the rumen contents if the opening is too large. used CO 2 The gas-purged wide-mouth collection bottle is used to collect the contents of the rumen, placed in a water bath at 39±0.5°C, and filtered with two layers, four layers, and six layers of gauze in sequence. The filtration should be as fast as possible to maintain the vitality of microorganisms. At the same time, CO is continuously passed into the obtaine...
Embodiment 2
[0033] In this example, soybean meal was used as raw material.
[0034] (1) Peel and crush the soybean meal, pass through a 40-mesh sieve, mix well, weigh 50g of soybean meal powder and put it into CO 2 Gas purged 500ml anaerobic fermentation bottle.
[0035] (2) When slaughtering ruminants such as cattle, sheep, deer, etc., quickly separate the rumen without opening the rumen, turn the rumen upside down several times, and mix the rumen contents evenly. Then cut the rumen with scissors, the opening of the rumen should not be too large, and it is inconvenient to collect the rumen contents if the opening is too large. used CO 2 The gas-purged wide-mouth collection bottle is used to collect the contents of the rumen, placed in a water bath at 39±0.5°C, and filtered with two layers, four layers, and six layers of gauze in sequence. The filtration should be as fast as possible to maintain the vitality of microorganisms. At the same time, CO is continuously passed into the obtain...
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Abstract
The invention relates to a preparation method of fermented vegetable protein, which is characterized by comprising the following steps: (1) crushing vegetable protein, and placing the crushed vegetable protein into an anaerobic fermentation container so as to crushed vegetable protein meal; (2) treating the rumen of a ruminant to obtain rumen liquid; (3) preparing mineral buffer solution; (4) inoculating the filtered liquid of the rumen into the vegetable protein meal, adding the mineral buffer solution and non-protein nitrogen, mixing uniformly, and introducing CO2 gas to remove oxygen; and (5) carrying out anaerobic fermentation for 24-84 hours under the temperature of 35-43 DEG C, and then drying under the temperature of 50-70 DEG C to obtain the fermented vegetable protein. Compared with the prior art, the preparation method is characterized in that waste resources of the ruminant are used as a bacteria source, the effect of removing main anti-nutritional factors in the vegetable protein is very obvious, trypsin restrains the high-efficiency degradation, the activity thereof is reduced by 42-63 percent, the content of phytic acid is reduced by 50-85 percent, the content of crude fiber is reduced by 10-45 percent, and the content of crude protein is increased by 10-20 percent. The content of the crude fiber has greater reduction, which is not easy to achieve in the prior art that adopting combined fermentation of dual strains or multiple strains to treat soya bean meal.
Description
technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for preparing fermented vegetable protein. Background technique [0002] With the rapid development of the global aquaculture industry, the shortage of feed ingredients, especially protein feed, is becoming increasingly obvious. As the most important source of high-quality protein in the aquafeed industry, fishmeal has a sharp increase in demand and a high price, which seriously restricts the development of aquaculture. Soybean meal and rapeseed meal are important plant-based protein sources with high nutritional value, relatively low price and wide sources, making them ideal alternative sources of fishmeal. However, these vegetable proteins often contain a variety of anti-nutritional factors, such as trypsin inhibitors, phytic acid and other anti-nutritional factors in soybean meal, which greatly limits the feeding value of soybean meal. Various processing metho...
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