Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of discriminating bovine, thus discriminated bovine and kit for discriminating bovine

A protein and wild-type technology, applied in the direction of food testing, biological testing, anti-animal/human immunoglobulin, etc., can solve the problems of backward application of proteomics analysis, achieve life improvement, life and stability, and increase production efficiency Effect

Active Publication Date: 2011-02-09
KINKI UNIVERSITY
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, the application of proteomic analysis in the field of animal husbandry is very backward, and the identification of proteins involved in the economic traits of beef cattle has not yet been realized, and the use of these proteins to identify cattle with useful economic traits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of discriminating bovine, thus discriminated bovine and kit for discriminating bovine
  • Method of discriminating bovine, thus discriminated bovine and kit for discriminating bovine
  • Method of discriminating bovine, thus discriminated bovine and kit for discriminating bovine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1. Exploration of proteins available as biomarkers

[0064] Extract the total protein (proteome) from multiple bovine white adipose tissue samples whose pedigree and meat quality are known, and search for proteins that can be used as biomarkers by correlating the proteome with the meat quality and other traits of each individual. Its details are as follows. In addition, unless otherwise stated, "%" below means "volume%".

[0065] (1) Protein extraction

[0066] As a sample of bovine white adipose tissue, white fat collected from individual Hida cattle whose economic characteristics such as pedigree, cut carcass weight, loin area, and pork belly thickness have been clarified, and which has been frozen and stored at the Livestock Experimental Field in Gifu Prefecture, Japan Tissue samples Approximately 150 sample materials.

[0067] Whole protein was extracted as described below. First, 1 ml of extraction solution (a solution formed by adding a proteolytic enzyme inhi...

Embodiment 2

[0084] 2. Identification of Proteins

[0085] For including figure 2 A plurality of protein spots (approximately 350 spots), including the protein spot surrounded by a circle, were extracted from the gel stained by two-dimensional electrophoresis, and identified by mass spectrometry. Specifically, follow the steps below.

[0086] (1) Extract from the gel

[0087] Use small tweezers to cut out the spots containing specific proteins from the gel, put the cut gel into the wells of a 96-well MTP plate, and put it in 0.1ml of decolorizing solution A (using methanol and 100mM ammonium bicarbonate aqueous solution, etc. 3 soaks of 20 minutes in the mixed solution). Remove the decolorizing solution A and soak in 0.1 ml of 100% acetonitrile for 5 minutes. The acetonitrile was evaporated and the gel was completely dried. Add 30 μl of trypsin solution (0.83 μg / ml trypsin (Sequencing grade Trypsin, manufactured by Promega), 25 mM ammonium bicarbonate) to the dried gel, and react ove...

Embodiment 3

[0093] 3. Data Analysis

[0094] Quantitative values ​​of protein spots obtained from two-dimensional electrophoresis results were managed using a laboratory information management system for proteomics analysis (BIOPRISM, manufactured by NEC Corporation), and were combined with various trait data (individual data, pedigree data, etc. 7 items, meat quality data 22 items) together to make a database, and analyze the correlation between each data.

[0095] In addition, the data of MS spectra and MS / MS spectra obtained by mass spectrometry were input into MASCOT (Matrix Science Corporation), for Swiss Prot (http: / / au.expasy.org / sprot / ) and NCBInr (http: / / au.expasy.org / sprot / ) and other public protein sequence databases, conduct peptide mass fingerprint (PMF) analysis, MS / MS ion search (IonSearch) analysis, and try to identify proteins. And, input the obtained results into the aforementioned database.

[0096] The data analysis results of the database show that the protein spot...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to View More

Abstract

Disclosed is a method of discriminating a bovine individual whereby proteins participating in the economical characteristics of beef cattle are identified by a proteomic analysis and a bovine individual having useful economical characteristics is discriminating with the use of these proteins as biomarkers. Also disclosed is a kit for discriminating a bovine individual to be used therein. The method of discriminating a bovine individual as described above comprises: (1) a collection step for collecting a body tissue from a bovine; (2) an extraction step for extracting all proteins from the body tissue thus collected; (3) a detection step for detecting wild type annexin A5 protein, an isoform of the wild type annexin A5 protein and a modified protein of the wild type annexin A5 protein or the isoform thereof that are contained in the all of the extracted proteins; and (4) a discrimination step for discriminating whether or not the average carcass weight is increased depending on the detected annexin A5 types. The wild type annexin A5 protein, the isoform thereof and modified proteins of the same are detected by using two-dimensional electrophoresis or an antigen-antibody reaction.

Description

technical field [0001] The present invention relates to a cattle discrimination method for discriminating cattle with excellent economic traits such as carcass weight, the cattle to be discriminated, and a cattle discrimination kit used in the method. Background technique [0002] At present, the breeding and improvement of beef cattle, especially the black-haired and Japanese-bred cattle, which are specialized carnivorous species peculiar to Japan, is carried out based on the statistical genetics breeding and improvement method based on the A method of estimating the heritability of cattle using the statistical genetic analysis of phenotype information and pedigree information accumulated so far as a platform. In addition, the establishment of the breeding improvement method has greatly contributed to the improvement of the heritability of Kurogewa species. [0003] However, this statistical genetics breeding improvement method is a method of estimating the probability (pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/447G01N33/48C07K16/18G01N33/53G01N33/12
CPCG01N2333/4718G01N33/68C07K16/18
Inventor 松本和也森本康一池上春香永井宏平
Owner KINKI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com