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Method for preparing microalgae polypeptide powder and microalgae polypeptide powder-containing flavoring

A technology of polypeptide powder and microalgae, which is applied in food preparation, application, food science, etc., can solve the problems of poor utilization of abundant protein and waste of resources, etc., to improve the grade and technical content, easy to control, The effect of high content

Active Publication Date: 2011-02-23
RUNKE BIOENG FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing deep processing of microalgae mainly focuses on the preparation of DHA microalgae oil. The algae meal after oil extraction is often treated as feed. The rich components, especially protein, are not well utilized, which is an invisible waste of resources.

Method used

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Examples

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Comparison scheme
Effect test

preparation example Construction

[0019] The preparation method of microalgae polypeptide powder of the present invention comprises the following steps:

[0020] (1) Crushing the algal meal, taking the algal meal powder and mixing it with water;

[0021] (2) Add protease to hydrolyze for 4-7 hours;

[0022] (3), inactivating the enzyme, and then concentrating the enzymatic solution;

[0023] (4) Add embedding agent, embedding, and finally spray drying to obtain microalgae polypeptide powder.

[0024] Wherein the addition of above-mentioned water is generally 8-12 times of the weight of algal meal powder. The enzymatic hydrolysis temperature of the protease is controlled at 45-60°C. The embedding agent mentioned above is β-cyclodextrin, modified starch or whey protein.

[0025] In order to make the enzymolysis conditions of the present invention milder and easy to control, while avoiding the production of carcinogen-chloropropanol in the acid hydrolysis process and avoiding the discharge of waste water and ...

Embodiment 1

[0028] Example 1: The preparation method of microalgae polypeptide powder of the present invention comprises the following steps:

[0029] (1) Crush the algae meal into powder, take the algae meal powder and add 8 times the amount of water to mix;

[0030] (2) At 45°C, first add 1‰ of peptide bond endonuclease (compound protease, Novozymes) for enzymolysis for 1 hour, and then add 3‰ of peptide bond exonuclease (flavored protease, Novozymes) Enzymolysis 6h;

[0031] (3) On the basis of the above, raise the temperature to 95°C, keep it warm for 30 minutes to inactivate the enzyme, and then concentrate in vacuo to obtain a concentrated enzymolysis solution, which is concentrated until the solid content is 20%-25% (that is, the weight of the solid content is the concentrated enzymolysis solution). 20%-25% of the weight of the concentrated enzymolysis solution), and then add modified starch accounting for 10% of the weight of the concentrated enzymolysis solution to the concent...

Embodiment 2

[0032] Example 2: The preparation method of microalgae polypeptide powder of the present invention comprises the following steps:

[0033] (1) Pulverize the algae meal, take the algae meal powder and add 10 times the amount of water to mix;

[0034] (2) At 50°C, first add 2‰ peptide bond endonuclease (papain A1, Guangxi Pangbo Biotechnology) for enzymolysis for 1 hour, then add 4‰ peptide bond exonuclease (flavor protease, Guangxi Pangbo Biotechnology) technology) enzymatic hydrolysis for 4h;

[0035] (3) Heat up to 95°C, keep warm for 30 minutes to inactivate the enzyme, then concentrate in vacuo to obtain a concentrated enzymolysis solution, and concentrate until the solid content is 20%-25% (that is, the weight of the solid is 20%-25% of the weight of the concentrated solution. %), then add whey protein accounting for 10% of the concentrated solution to the concentrated enzymolysis solution, and then embed at 90°C for 1 hour, control the air inlet temperature at 180-200°...

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Abstract

The present invention relates to a microalgae polypeptide powder-containing flavoring with rich nutrition, and a preparation method thereof. The method comprises the following steps of: crushing microalgae meal, adding the microalgae meal into water and mixing; adding protease and performing enzymolysis for 4 to 7 hours; and deactivating enzyme, concentrating enzymolysis solution, adding an embedding medium, embedding and finally performing spray-drying to obtain the microalgae polypeptide powder. Because the microalgae meal is used as the raw material, and high molecular protein in the microalgae meal is enzymolyzed into polypeptide and amino acid which can be easily absorbed by human bodies by using biological engineering enzymolysis technology, the produced product has high content of protein and amino acid, rich nutrition and high freshness and contains docosahexaenoic acid (DHA) microalgae oil; the product can be used for an flavoring type essence reaction essence group and used for the industries such as biological pharmacy, health-care products, household flavorings, instant noodle flavorings, puffed foods, baked foods, seafood processed products and the like, and is a good method for improving the added value of the microalgae meal.

Description

technical field [0001] The invention relates to a microalgae deep processing technology, in particular to a method for preparing algae dregs into microalgae polypeptide powder and seasoning containing the microalgae polypeptide powder. Background technique [0002] Microalgae is a nutritious and balanced algae that is beneficial to human health. It is not only rich in DHA, but also rich in protein, amino acids, polysaccharides and other nutrients. [0003] Algae meal is the residue of oil refined by microalgae. Its main components are protein and amino acid, and it contains a certain amount of DHA algae oil, which has high nutritional value and medicinal value. The existing deep processing of microalgae mainly focuses on the preparation of DHA microalgae oil. The algae meal after oil extraction is often treated as feed. The rich components, especially protein, are not well utilized, which is an invisible waste of resources. Contents of the invention [0004] The purpose o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/337A23L1/22A23L1/29A23L33/00A23L17/60A23L27/00
Inventor 李建平司华静彭晓芳姜悦
Owner RUNKE BIOENG FUJIAN
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