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PCR (Polymerase Chain Reaction) kit for detecting CYP2C19 genotyping

A technology of CYP2C19 and genotyping, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high price, high false positive, low throughput, etc., and achieve low price, high specificity, high The effect of flux detection

Active Publication Date: 2011-03-23
SUZHOU KUANGYUAN MOLECULAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The purpose is to solve the problems of high price, low efficiency, low throughput and high false positive when using PCR amplification method to detect CYP2C19 genotype

Method used

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  • PCR (Polymerase Chain Reaction) kit for detecting CYP2C19 genotyping
  • PCR (Polymerase Chain Reaction) kit for detecting CYP2C19 genotyping
  • PCR (Polymerase Chain Reaction) kit for detecting CYP2C19 genotyping

Examples

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Embodiment 1

[0043] Example 1: A PCR kit for detecting CYP2C19 genotyping

[0044] A PCR kit for detecting CYP2C19 genotyping comprising:

[0045] 10×PCR Buffer;

[0046] dNTPs (2.5mM);

[0047] Pfu DNA polymerase (2.5U / μl);

[0048] Primers (10mM each);

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Abstract

The invention discloses a PCR kit for detecting CYP2C19 genotyping. In the invention, a series of modified detection primers and high-fidelity DNA polymerase mediated PCR are adopted to detect the gene CYP2C19*1 / *2 type (namely, the 681 G / A locus) and / or the CYP2C19*1 / *3 type (namely, the 636 G / A locus). The kit of the invention can realize that the base types of the two locuses influencing the activity of drug metablic enzyme coded by the CYP2C19 gene in the CYP2C19 gene with low cost, high efficiency and high flux. The mutation of the two locuses can explain the weak metabolism of Chinese people for associated drugs, and the detection result of the kit of the invention can be used for instructing the individual drug.

Description

technical field [0001] The invention relates to a PCR kit for detecting CYP2C19 genotyping, in particular to a method for detecting two sites of the cytochrome P450 enzyme 2C19 gene that affects the metabolism of commonly used clinical drugs by using the polymerase chain reaction (PCR) method Genotype, namely CYP2C19 *1 / *2 type (681G / A) and CYP2C19 *1 / *3 type (636 G / A) kit, belongs to the clinical molecular detection technology in the field of biomedicine. Background technique [0002] Cytochrome P450 (CytochromeP450, CYP450) is an isoenzyme encoded by a group of structurally and functionally related superfamily genes (Superfamily gene). For humans, it mainly exists in liver cell microsomes. In the CYP450 superfamily, CYP2 is the largest family with 15 subfamilies (CYP2A to 2Q), which metabolize about 20% of the drugs currently in clinical use, among which CYP2A, CYP2C, CYP2D and CYP2E all have genetic polymorphisms and races difference. CYP2C accounts for about 20% in h...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王进秦正红杨舒婷何晓辉
Owner SUZHOU KUANGYUAN MOLECULAR BIOTECH
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