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Bifidobacterium animalis and application thereof in preparing bifidobacterium fermented beverage

An animal bifidobacteria, bifidobacteria technology, applied in bacteria, food science and other directions, can solve the problem of bifidobacteria beverages or preparations on the market

Active Publication Date: 2011-04-13
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no bifidobacterium drink or preparation using yacon as raw material on the market

Method used

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  • Bifidobacterium animalis and application thereof in preparing bifidobacterium fermented beverage
  • Bifidobacterium animalis and application thereof in preparing bifidobacterium fermented beverage
  • Bifidobacterium animalis and application thereof in preparing bifidobacterium fermented beverage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Obtaining of Bifidobacterium animalis 01CGMCC 1353

[0037] 1. The acquisition and identification of Bifidobacterium animalis 01CGMCC 1353

[0038] 25g of fresh feces from long-lived elderly people in Bama, Guangxi were obtained under sterile anaerobic conditions, and placed in 225mL of sterile pre-reduced normal saline. Mix well, and use the Hengate anaerobic dilution technique to perform 10-fold serial dilution of the sample, and select the dilution factor as 10 5 、10 6 、10 7 Separation was carried out by the Hungate anaerobic rolling tube method, and cultured in a 37°C incubator for 48-72h. Select small and medium-sized colonies characterized by smooth, convex, neat edges, milky white or slightly yellow, and soft texture, inoculate them in MRS liquid medium, culture anaerobically for 24-48 hours, and perform Gram staining. Select colonies with bifidobacteria morphology and other characteristics, and then carry out aerobic and anaerobic culture, select ...

Embodiment 2

[0083] Embodiment 2, utilize bifidobacterium animalis (Bifidobacterium animalis) 01CGMCC 1353 to prepare yacon fruit fermented beverage

[0084] The present invention utilizes the bifidobacterium animalis (Bifidobacterium animalis) 01CGMCC1353 that embodiment 1 isolates to prepare the yacon bifidobacterium fermented beverage, and the preparation method is as follows:

[0085] 1. Preparation of Bifidobacterium Seeds

[0086] 1) Preparation of first-generation seeds

[0087] Bifidobacterium animalis 01CGMCC 1353 was inoculated into MRS liquid medium, and anaerobically cultured at 37° C. for 24h-48h to obtain first generation seeds.

[0088] 2) Preparation of second generation seeds

[0089] The first generation of seeds were inoculated in MRS liquid medium for anaerobic culture at 37°C for 14-16h (to the end of the logarithmic phase) (the end of the logarithmic phase is a transition period when the logarithmic phase is about to end and enters the mature phase. By measuring the...

Embodiment 3

[0103] The preparation of embodiment 3 bifidobacteria snow lotus fruit juice

[0104] 1. Preparation of Snow Lotus Juice

[0105] 1) washing and cutting the yacon fruit, blanching in water at 85-100°C to eliminate enzymes, then removing the yacon pulp, adding water at a mass ratio of 1:1 between the yacon pulp and water, mixing and beating to obtain yacon pulp;

[0106] 2) Add 5% skim reconstituted milk of the volume of yacon pulp (preparation method of skim reconstituted milk is 10-12.5g of skim milk is added to 1L of drinking water, fully stirred to dissolve) and 8% tomato juice is 8% of the volume of yacon pulp, and then Carry out homogenization for the first time under 8Mpa condition with homogenizer (Langfang Huitong Food Machinery Company, Huitong 25Mpa, 40Mpa homogenizer), carry out homogenization for the second time under 12Mpa condition, obtain mixed fruit juice;

[0107] 3) Fill the mixed fruit juice with nitrogen to reduce the redox potential to below -0.03ev, and ...

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Abstract

The invention discloses a bifidobacterium animalis and application thereof in preparing a bifidobacterium fermented beverage. The bifidobacterium animalis is bifidobacterium animalis 01, and has the preservation number of CGMCC 1353. The bifidobacterium animalis 01 with the preservation number of CGMCC 1353 can be used for preparing the bifidobacterium fermented beverage. The prepared yacon bifidobacterium fermented beverage has mild color (as shown in figure 1), good mouth feel, good flavor and longer shelf life, and is a health beverage with a good health-care effect.

Description

technical field [0001] The invention relates to an animal bifidobacterium strain and its application in the preparation of bifidobacterium fermented beverages, in particular to a process for preparing fermented beverages from an animal bifidobacterium strain and its fermented yacon compound juice. Background technique [0002] There are tens of billions of bacteria in the human intestinal tract. Most of these bacteria are beneficial and harmless to coexist peacefully with people. They are called the normal flora of the human body. The total weight of human intestinal flora accounts for about 1 / 50-1 / 60 of the total weight of the human body, and more than 95% of them are live bacteria, which carry out active growth and metabolism activities in the human intestinal tract every day. It can be called the sixth largest organ in the human body. Human nutrition, digestion, biological antagonism, immunity, anti-tumor, drug efficacy and anti-aging are closely related to human intesti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L2/84
Inventor 李平兰王洋旭日花刘国荣
Owner 哈尔滨美华生物技术股份有限公司
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