Fluorescent signal detection device of real-time fluorescent quantitative PCR (Polymerase Chain Reaction) instrument

Abstract

The invention discloses a fluorescent signal detection device of a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) instrument. One end of a screw rod is fixedly connected with a transmission device through a bearing arranged on a guide rail; a slide block is in sliding fit with the guide rail, arranged together with the screw rod and fixedly connected with a second stepping motor; light rays emitted by a light source enter a sample to be detected sequentially through a two-phase color spectroscope, a plane mirror, a lens and a light emitting hole in a light path module shell; a fluorescent group contained in the sample to be detected is stimulated to generate fluorescent light, and the fluorescent light roundly enters the receiving end of a photomuitplier through the light emitting hole, the lens, the plane mirror, the two-phase color spectroscope and a filter lens; an output shaft of the second stepping motor is fixed with the light path module shell; the pulse signal input end and the direction signal input end of a first subdivision driver and a second subdivision driver are connected with the corresponding output end of a controller; the output end of the first subdivision driver is connected with the transmission device; and the output end of the second subdivision driver is connected with the second stepping motor. The fluorescent signal detection device has compact structure and simple and reliable light path system.

Description

technical field [0001] The invention relates to the detection of biology and medicine, in particular to a fluorescence signal detection device of a real-time fluorescence quantitative PCR instrument. Background technique [0002] Real-time fluorescence quantitative PCR technology can realize quantitative analysis of DNA templates, which is of great significance to molecular biology research and medical research. The fluorescence detection device of the real-time fluorescence quantitative PCR instrument is one of the cores of the real-time fluorescence quantitative PCR instrument. The traditional fluorescence detection device has a large mechanical structure, complex optical path and high price, which makes the real-time fluorescence quantitative PCR instrument popularization rate low, and only a few have the strength. The purchase of large companies, large enterprises and national large or key experimental units affects the development of scientific research and practical ap...

AI Technical Summary

Problems solved by technology

The fluorescence detection device of the real-time fluorescent quantitative PCR instrument is one of the cores of the real-time fluorescent quantitative PCR instrument. The traditional fluorescent detection device has a huge mechanical structure, complex optical path, and high price, which makes the real-time fluorescent quantitative PCR instrument popular. Only a few have the strength Large companies, large enterprises and national large-scale or key experimental units purchase, which affects the development of scientific research and practical application in this field

At present, real-time fluorescence quantitative PCR instruments mostly use single-chip microcomputers to realize motion control, but single-chip microcomputers have slow processing speed, few functions, and cannot design a friendly human-machine interface. More importantly, their storage space is relatively small, and large programs cannot be run.

In addition to the fluorescence detection device, the real-time fluorescent quantitative PCR instrument also includes a temperature control device. The complex system will inevitably make the software and algorithm of the real-time fluorescent quantitative PCR instrument complicated. Using a single-chip microcomputer to realize motion control will make the real-time fluorescent quantitative PCR instrument The overall functionality and performance of the

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