Fluorescent signal detection device of real-time fluorescent quantitative PCR (Polymerase Chain Reaction) instrument

Abstract

The invention discloses a fluorescent signal detection device of a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) instrument. One end of a screw rod is fixedly connected with a transmission device through a bearing arranged on a guide rail; a slide block is in sliding fit with the guide rail, arranged together with the screw rod and fixedly connected with a second stepping motor; light rays emitted by a light source enter a sample to be detected sequentially through a two-phase color spectroscope, a plane mirror, a lens and a light emitting hole in a light path module shell; a fluorescent group contained in the sample to be detected is stimulated to generate fluorescent light, and the fluorescent light roundly enters the receiving end of a photomuitplier through the light emitting hole, the lens, the plane mirror, the two-phase color spectroscope and a filter lens; an output shaft of the second stepping motor is fixed with the light path module shell; the pulse signal input end and the direction signal input end of a first subdivision driver and a second subdivision driver are connected with the corresponding output end of a controller; the output end of the first subdivision driver is connected with the transmission device; and the output end of the second subdivision driver is connected with the second stepping motor. The fluorescent signal detection device has compact structure and simple and reliable light path system.

Description

technical field [0001] The invention relates to the detection of biology and medicine, in particular to a fluorescence signal detection device of a real-time fluorescence quantitative PCR instrument. Background technique [0002] Real-time fluorescence quantitative PCR technology can realize quantitative analysis of DNA templates, which is of great significance to molecular biology research and medical research. The fluorescence detection device of the real-time fluorescence quantitative PCR instrument is one of the cores of the real-time fluorescence quantitative PCR instrument. The traditional fluorescence detection device has a large mechanical structure, complex optical path and high price, which makes the real-time fluorescence quantitative PCR instrument popularization rate low, and only a few have the strength. The purchase of large companies, large enterprises and national large or key experimental units affects the development of scientific research and practical ap...

Images