Method for rapidly accumulating micro-algae intracellular grease

A microalgae and oil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of no large-scale application value, inability to scale up, inability to achieve aseptic culture, etc.

Inactive Publication Date: 2011-04-20
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The biggest problem with this model is that the light-induced culture process cannot be axenic after being scaled up (Scott SA, Davey MP, Dennis JS, et al. Biodiesel from algae: challenges and prospects. Current Opinion in Biotechnology, 2010, 21: 1- 10), since the heterotrophic culture of microalgae requires that the algae must not contain any bacteria, this model cannot be scaled up and has no large-scale application value

Method used

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  • Method for rapidly accumulating micro-algae intracellular grease
  • Method for rapidly accumulating micro-algae intracellular grease
  • Method for rapidly accumulating micro-algae intracellular grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Add the following heterotrophic medium and tap water to 25 L in a 50 L bioreactor and then sterilize it. When the temperature drops to 30° C., add Chlorella pyrenoidosa according to 12% of the working volume to start heterotrophic culture.

[0104] Heterotrophic culture conditions: the temperature is 30°C, the initial speed is 150r / min, the air flow is 1vvm, the pH is less than 8.0, and the dissolved oxygen is controlled to be more than 15% by adjusting the speed during the cultivation process.

[0105] Dilute the high-density algae liquid that has run out of glucose during the heterotrophic culture to about 2.70g / L, add the following light-induced medium, and transfer it to a 3L flat-plate photoreactor for light-induced culture at a temperature of 30°C. Strong 8000lx, air flow is 1vvm. After light-induced culture for 12 hours, the cell density decreased from 2.70g / L to 2.65g / L, and the oil content increased from 9.70% to 19.70% (see figure 1 ).

[0106] Heterotrophic...

Embodiment 2

[0117] Add heterotrophic medium and tap water to 2.5T in a 5T fermenter and then sterilize it. When the temperature drops to 30°C, use a 500L fermenter as a seed tank to cultivate Chlorella vulgaris. The level of dissolved oxygen in the liquid is used to adjust the tank pressure, ventilation and stirring speed, maintain sufficient oxygen content in the culture liquid, and ensure the rapid growth of algae cells.

[0118] After diluting the high-density algae liquid that had run out of glucose in the previous fed-batch heterotrophic culture process, it was transferred to a 30L flat-plate photoreactor and a 60L bubbling large pot for light-induced culture outdoors. Light-induced culture conditions: natural temperature, temperature at 13-30°C, natural light, light intensity at 0-36klx (see figure 2 ), the air flow rate is 1vvm. After 8 hours of light-induced culture, the algal cell density in the 30L plate reactor decreased from 1.60g / L to 1.46g / L, and the oil content increased ...

Embodiment 3

[0131] Example 3: Research on the Changes of the Main Biochemical Components of Chlorella ellipsoides During Heterotrophic-Dilution-Light-Induced Series Culture

[0132] In this example, the changes of the main biochemical components in the heterotrophic-dilution-light-induced series culture process of Chlorella ellipsoides were measured at the level of a 500mL shake flask / 3L cylindrical photobioreactor.

[0133] Cultivate Chlorella ellipsoides in a manner similar to Examples 1 and 2 with heterotrophic-dilution-light-induced serial culture technology, 500mL shake flask, liquid volume 200mL, 28°C, 150rpm, until the algae cell density reaches above 10g / L And when the glucose is exhausted, transfer to a 3L cylindrical photobioreactor for light-induced culture, the algae cell density is about 2g / L, the temperature is 30°C, and the light intensity is 10Klx. The medium used for heterotrophy and light induction is the same as the corresponding medium for Chlorella pyrenoidosa. At th...

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Abstract

The invention relates to a method for rapidly accumulating micro-algae intracellular grease, which comprises steps of heterotrophic culturing, dilution, photo-inductive culturing, micro-algae collection and grease extraction and the like. By using the method, advantages of rapid micro-algae intracellular grease accumulation in the photo-inductive stage are brought into full play so as to provide significant technical means for solving the problem of exceeded cost caused by insufficient raw materials in process of preparing biological fuels (such as diesel oil, aviation kerosene and the like) in a large scale.

Description

technical field [0001] The invention belongs to the field of bioenergy and / or the field of microalgae biotechnology, and relates to a method for rapidly accumulating oil in microalgae cells. Background technique [0002] Microalgae energy has broad prospects and unique advantages, which have been recognized both at home and abroad. So far, the research and development work in this field in all countries in the world is still in the initial stage of experimental research and pilot test demonstration (Li Yuanguang, Tan Tianwei, Huang Yingming, Some scientific problems and analysis in the industrialization technology of microalgae biodiesel, Basic Science of China, 2009, May, 64-70), but they all encountered the bottleneck of high cost due to immature technology, so microalgae energy has not yet been produced on a large scale in the world. [0003] Mastering the efficient culture mode of energy microalgae is the basis for realizing the large-scale production of microalgae ener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/12C12R1/89
Inventor 李元广范建华王伟良黄建科李淑兰魏鸿刚沈国敏李际军
Owner EAST CHINA UNIV OF SCI & TECH
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