Wuyi Mountain rock tea extract, and preparation method and application thereof

A technology of extract and rock tea, applied in Wuyishan rock tea extract and its preparation and application fields, can solve the problems of low solubility, reduced antioxidant capacity, insufficient biological activity and the like

Active Publication Date: 2011-05-11
上海市新文达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, catechin, the main component of tea polyphenols, is very active in nature and is easily oxidized under air and acidic conditions, resulting in a decrease in its antioxidant capacity.
And from the structural point of view, it has both a hydrophobic benzene ring and a hydrophilic hydroxyl group, so the molecule is both hydrophilic and lipophilic, but its solubility in water and oil phases is not high. As a result, its biological activity cannot be fully exerted, which affects its application in food, medicine and other fields
[0007] However, there is no direct extraction and separation of tea polyphenols containing glycosides from natural plant rock tea, such as "Dahongpao" oolong tea, etc.

Method used

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  • Wuyi Mountain rock tea extract, and preparation method and application thereof
  • Wuyi Mountain rock tea extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Weigh 1000g of rock tea Dahongpao, crush it, 20-100 meshes, add 6000ml of ethanol aqueous solution with a volume concentration of 80%, rotate and extract at 65°C for 2 hours, filter to obtain the extract, and continue to use 6000ml of an ethanol aqueous solution with a volume concentration of 80%. Rotate extraction at 65°C for 2 hours, filter, combine two extracts, decolorize, filter, concentrate the filtrate to no ethanol, centrifuge, filter, take the filtrate to pass through a macroporous adsorption resin column, first rinse with 3000ml of distilled water, and then with a volume concentration of 30 %, 50%, 70%, and 90% ethanol aqueous solutions were eluted in sequence with 2500ml each, and the eluents were collected separately, each fraction was 150ml, and analyzed by silica gel thin-layer chromatography respectively, and the same fractions were combined, and the 11th to 25th Fractions, whose Rf value is 0.16-0.45, are concentrated to a certain amount, sequentially ext...

Embodiment 2

[0062] Weigh 1000g of rock tea Dahongpao, crush it, 20-100 meshes, add 6000ml of ethanol aqueous solution with a volume concentration of 70%, rotate and extract at 65°C for 2 hours, filter to obtain the extract, and continue to use 6000ml of an ethanol aqueous solution with a volume concentration of 70%. Rotate extraction at 65°C for 2 hours, filter, combine two extracts, decolorize, filter, concentrate the filtrate to no ethanol, centrifuge, filter, take the filtrate to pass through a macroporous adsorption resin column, first rinse with 3000ml of distilled water, and then with a volume concentration of 30 %, 50%, 70%, and 90% ethanol aqueous solutions were eluted in sequence with 2500ml each, and the eluents were collected separately, each fraction was 150ml, and analyzed by silica gel thin-layer chromatography respectively, and the same fractions were combined, and the 11th to 25th The fraction, whose Rf value is 0.16-0.45, is concentrated to a certain amount, and extracted ...

Embodiment 3

[0064] Weigh 1000g of rock tea Dahongpao, crush it, 20-100 mesh, add 6000ml of ethanol aqueous solution with a volume concentration of 50%, rotate and extract at 65°C for 2 hours, filter to obtain the extract, and continue to use 6000ml of ethanol solution with a volume concentration of 50% for the filter residue, Rotate extraction at 65°C for 2 hours, filter, combine two extracts, decolorize, filter, concentrate the filtrate to no ethanol, centrifuge, filter, take the filtrate to pass through a macroporous adsorption resin column, first rinse with 3000ml of distilled water, and then with a volume concentration of 30 %, 50%, 70%, and 90% ethanol aqueous solutions were eluted in sequence with 2500ml each, and the eluents were collected separately, each fraction was 150ml, and analyzed by silica gel thin-layer chromatography respectively, and the same fractions were combined, and the 11th to 25th Fractions, whose Rf value is 0.16-0.45, are concentrated to a certain amount, sequen...

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Abstract

The invention discloses a method for preparing a Wuyi Mountain rock tea extract. The method comprises the following steps of: (1) extracting the Wuyi Mountain rock tea by using ethanol aqueous solution; and (2) separating the substance obtained in the step (1) by using a macroporous resin column and collecting a fraction with the volume ratio of developing agent dichloromethane to methanol to water of 8:3:1 and an Rf value of 0.16 to 0.45 during detection by silica gel thin layer chromatography so as to prepare the extract. The method can also comprise the following steps of: performing chromatographic separation on the substance obtained in the step (2) by using a silica gel column; and collecting one or more of three fractions with the volume ratio of the developing agent dichloromethane to the methanol to the water of 8:3:1 and the Rf value of 0.44 during detection by silica gel thin layer chromatography. The invention also relates to the Wuyi Mountain rock tea extract and application thereof. The Wuyi Mountain rock tea extract has stable properties and high water solubility. Compared with the conventional tea polyphenol aglycon, the Wuyi Mountain rock tea extract has more stable properties and higher activity.

Description

technical field [0001] The present invention specifically relates to a kind of Wuyi mountain rock tea extract and its preparation method and application. Background technique [0002] Wuyi Mountain Rock Tea (Wuyi Mountain Rock Tea) has a long history and was planted as early as the beginning of the second century. Wuyi Mountain Rock Tea (Wuyi Mountain Rock Tea) has the fragrance of green tea and the sweetness of black tea. Tea is the best among teas, and there are many varieties, such as famous teas such as Dahongpao, Oolong, Tieguanyin, Baijiguan, and Shuijingui. "Dahongpao" (camellia.sinensiscv.dahongpao) is the most valuable and enjoys the reputation of "king of tea". Dahongpao grows on the mid-mountain cliff of Jiulongcao in Wuyi Mountain Scenic Area, where there are mountains surrounded by mountains and mountains, secluded streams with clear water and long streams, and clouds and fog all the year round. Wuyi Mountain belongs to the "Danxia Terrain", which is formed by...

Claims

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Application Information

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IPC IPC(8): A23F3/18A61K36/185A61K8/97A61P39/06A61P3/10A61P25/00A61P17/00A61Q19/02
Inventor 刘桂云骆峰骆滨
Owner 上海市新文达生物科技有限公司
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