Wuyi Mountain rock tea extract, and preparation method and application thereof
A technology of extract and rock tea, applied in Wuyishan rock tea extract and its preparation and application fields, can solve the problems of low solubility, reduced antioxidant capacity, insufficient biological activity and the like
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Embodiment 1
[0060] Weigh 1000g of rock tea Dahongpao, crush it, 20-100 meshes, add 6000ml of ethanol aqueous solution with a volume concentration of 80%, rotate and extract at 65°C for 2 hours, filter to obtain the extract, and continue to use 6000ml of an ethanol aqueous solution with a volume concentration of 80%. Rotate extraction at 65°C for 2 hours, filter, combine two extracts, decolorize, filter, concentrate the filtrate to no ethanol, centrifuge, filter, take the filtrate to pass through a macroporous adsorption resin column, first rinse with 3000ml of distilled water, and then with a volume concentration of 30 %, 50%, 70%, and 90% ethanol aqueous solutions were eluted in sequence with 2500ml each, and the eluents were collected separately, each fraction was 150ml, and analyzed by silica gel thin-layer chromatography respectively, and the same fractions were combined, and the 11th to 25th Fractions, whose Rf value is 0.16-0.45, are concentrated to a certain amount, sequentially ext...
Embodiment 2
[0062] Weigh 1000g of rock tea Dahongpao, crush it, 20-100 meshes, add 6000ml of ethanol aqueous solution with a volume concentration of 70%, rotate and extract at 65°C for 2 hours, filter to obtain the extract, and continue to use 6000ml of an ethanol aqueous solution with a volume concentration of 70%. Rotate extraction at 65°C for 2 hours, filter, combine two extracts, decolorize, filter, concentrate the filtrate to no ethanol, centrifuge, filter, take the filtrate to pass through a macroporous adsorption resin column, first rinse with 3000ml of distilled water, and then with a volume concentration of 30 %, 50%, 70%, and 90% ethanol aqueous solutions were eluted in sequence with 2500ml each, and the eluents were collected separately, each fraction was 150ml, and analyzed by silica gel thin-layer chromatography respectively, and the same fractions were combined, and the 11th to 25th The fraction, whose Rf value is 0.16-0.45, is concentrated to a certain amount, and extracted ...
Embodiment 3
[0064] Weigh 1000g of rock tea Dahongpao, crush it, 20-100 mesh, add 6000ml of ethanol aqueous solution with a volume concentration of 50%, rotate and extract at 65°C for 2 hours, filter to obtain the extract, and continue to use 6000ml of ethanol solution with a volume concentration of 50% for the filter residue, Rotate extraction at 65°C for 2 hours, filter, combine two extracts, decolorize, filter, concentrate the filtrate to no ethanol, centrifuge, filter, take the filtrate to pass through a macroporous adsorption resin column, first rinse with 3000ml of distilled water, and then with a volume concentration of 30 %, 50%, 70%, and 90% ethanol aqueous solutions were eluted in sequence with 2500ml each, and the eluents were collected separately, each fraction was 150ml, and analyzed by silica gel thin-layer chromatography respectively, and the same fractions were combined, and the 11th to 25th Fractions, whose Rf value is 0.16-0.45, are concentrated to a certain amount, sequen...
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