Oligomeric proanthocyanidins and method for extracting same

An oligomeric proanthocyanidin, extraction technology, applied in chemical instruments and methods, pharmaceutical formulations, antidote, etc., can solve problems such as large differences in product purity

Active Publication Date: 2013-05-08
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its products can basically only control total anthocyanine (pigment) pigments. Due to differences in concepts and detection methods, the purity of products advertised by different companies varies greatly, ranging from 25% to 98%, but in fact they are all more Tangerine anthocyanin crude extract, high-quality oligomeric proanthocyanidin products are still rare

Method used

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  • Oligomeric proanthocyanidins and method for extracting same
  • Oligomeric proanthocyanidins and method for extracting same
  • Oligomeric proanthocyanidins and method for extracting same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Extraction Proanthocyanidins in lingonberries (ambient temperature of 4°C)

[0064] The operation process in this embodiment 1 was all carried out at an ambient temperature of 4° C. (using an air conditioner to control the temperature).

[0065] After the Daxing'anling Dusi lingonberry was squeezed to remove the juice, weigh 100g of the pomace, add 300ml of pre-cooled (4°C) 70% v / v acetone to extract for 1h, centrifuge at 4000rpm for 30min, collect the supernatant filtered filtrate, concentrate in vacuo, and then Put the concentrated solution on the column (the column filler is HP-2MG resin, 4° C.), and flush the column volume with deoxygenated water (distilled water) with nitrogen gas for 1 to 2 column volumes before loading the column. Put the concentrated solution on the column until the HP-2MG resin is saturated. After the sample is loaded, it is eluted with deoxygenated distilled water with pH=3.0 until the washing solution has no obvious red color (OPC...

Embodiment 2

[0089] Embodiment 2 extracts Proanthocyanidins in lingonberries (ambient temperature of 16°C)

[0090] The operation process in this embodiment 2 is all carried out under the environment temperature of 16 ℃ (using air conditioner temperature control). Other conditions and steps and raw material are completely consistent with embodiment 1. After testing, the obtained cranberry proanthocyanidin product is basically consistent with that of Example 1, the proanthocyanidin content is 95% (w / w), and the content of proanthocyanidin (including monomers) below the tetramer reaches 90% (w / w).

Embodiment 3

[0092] The conditions and steps of the method for extracting proanthocyanidins used in this example, as well as the raw materials, and the methods for determining total polyphenols, proanthocyanidins and total flavanols are consistent with those in Example 1.

[0093] 1.1 Determination of leaching solvent (except that the solvent used is different, all the other method conditions and raw materials are the same as in Example 1)

[0094] Methanol, ethanol, and acetone are three commonly used extractants for phenolic substances. Select the same concentration of ethanol, methanol, and acetone to extract, determine the best extraction solvent, and the results are obtained by figure 2 It can be seen that the extraction effect of methanol and acetone on polyphenols is better than that of ethanol, and it is not easy to introduce impurities. Methanol is highly toxic. Therefore, acetone was selected as the best extraction solvent in this experiment.

[0095] 1.2 Single factor experi...

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Abstract

The invention provides a method for extracting oligomeric proanthocyanidins (OPC) from OPC-containing plant sources. The method is characterized in that: in the extracting process, conditions of no oxygen or low oxygen and low temperature are kept and a lightproof environment is used. The invention also provides the oligomeric proanthocyanidins extracted by the method; and the oligomeric proanthocyanidins is characterized by being rich in proanthocyanidins oligomers, and the extract can be auto-oxidized under the natural condition. The invention also provides application of proanthocyanidins products prepared by the method in removing superoxide anion radicals O2- and hydroxyl radicals HO.

Description

technical field [0001] The present invention relates to a kind of oligomeric proanthocyanidin and its extraction method, especially to the method of high activity proanthocyanidin obtained by solvent extraction and column chromatography and the oligomeric proanthocyanidin obtained by the method under the conditions of avoiding light, deoxidation and low temperature. Proanthocyanidin products. Background technique [0002] 1. The function of proanthocyanidins [0003] Proanthocyanidins are a kind of flavanol polyphenolic compounds abundantly present in lingonberries, grape seeds and other fruits. It is also the most important functional component in lingonberry. The main coloring substances are delphinidin (De), cyanidin (Cy), petunidin (Pet), peonidin (Peo) and malvidin (malvidin). ; Mal) composed of numerous anthocyanins and acetylated anthocyanins derived from five pigments, and the types and contents of anthocyanins vary depending on the lingonberry variety. Bilberry ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62A61K31/352C09K15/08A61P39/06
Inventor 董先智孙波陈少云刘翠
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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