Method for producing low-temperature phytase by fermenting microorganisms

Abstract

The invention discloses a method for producing low-temperature phytase by fermenting microorganisms. The method comprises the steps of: performing low-temperature domestication on microorganisms producing phytase step by step to ensure that the microorganisms grow well in low-temperature environment; performing amplification culture on phytase-producing bacteria obtained after low-temperature domestication step by step at a temperature of between 12 and 18 DEG C, inoculating into a liquid fermentation culture medium according to the inoculation amount which is 4 to 10 percent of the volume offermentation liquor, and culturing for 72 to 144h at a temperature of between 12 and 18 DEG C, namely finishing the production of the low-temperature phytase by fermenting the microorganisms; centrifuging the fermentation liquor at a rotating speed of between 6,000 and 10,000rpm to collect liquid, wherein the collected liquid is crude enzyme fluid; and further concentrating, separating and purifying the crude enzyme fluid according to different needs and different using objects to obtain enzyme preparations with different activities, purities and formulations.

Description

technical field [0001] The invention relates to the fields of microbiology, enzyme engineering, fermentation engineering, biochemistry and the like, in particular to a low-temperature phytase microbial fermentation production method. The low-temperature phytase produced by the invention is mainly used in feed industry, food processing, environmental protection, medicine and other fields. It can improve the utilization rate, conversion rate and productivity of raw materials, reduce production costs, and improve and enhance product quality. Background technique [0002] Phytase is a phytic acid hydrolase, which can catalyze the hydrolysis reaction to release phosphate from phytic acid, promote the absorption and utilization of mineral elements by animals, and more importantly, improve the utilization rate of phosphorus and reduce phosphorus pollution in the environment ( Huang Zunxi et al., 1999). In addition, phytase is also a new type of food additive and a catalyst for th...

AI Technical Summary

Problems solved by technology

Most of the currently used phytases are medium and high-temperature type phytases (Zhang Ruohan et al., 1996; Luis Pasamonts et al, 1997). The research on low-temperature phytases is still in its infancy, mainly focusing on strain selection and fermentation. Condition optimization, enzymatic properties, and related gene cloning; however, the industrialization, large-scale production and application of low-temperature phytase have not been reported yet.