Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of effective ingredient of rosewood heart wood

An effective component and aroma-reducing technology is applied in the field of traditional Chinese medicine extracts for the treatment of cardiovascular diseases, which can solve the problems of delayed blood cessation and weak, and achieve the effects of easy quality control, reasonable preparation method and reasonable design.

Inactive Publication Date: 2011-07-13
ZHEJIANG UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, Jiangxiang is one of the components of Guanxin No. Ⅱ prescription. Modern pharmacological experiments have shown that Jiangxiang alone can significantly promote the recovery of blood flow in the experimental mesenteric microcirculation of mice, as well as the recovery of arterioles after constriction and local microcirculation. recovery, its anti-adrenaline-induced contraction of arterioles is stronger, and its effect on delaying blood stop is weaker

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of effective ingredient of rosewood heart wood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 Preparation of effective components of balm

[0014] Add ethyl acetate and ethanol after crushing the balsamic medicinal material, heat and extract to obtain extract I, discard it. Add the medicinal dregs to ethanol aqueous solution, heat and extract to obtain the extract II, concentrate the extract II into an extract, dissolve the extract with 50% methanol, and use ODS-C 18 Column to separate it, first use low-concentration methanol as mobile phase to obtain eluent I, then use high-concentration methanol as mobile phase to obtain eluent II, obtain sample after concentration and drying; continue separation with preparative liquid chromatography The obtained sample was separated by preparative liquid chromatography, and the solution was collected in a period of 48.2-53.8 minutes, concentrated and dried to obtain effective components.

Embodiment 2

[0015] Example 2 Preparation of effective components of balm

[0016] Take 250 g of the medicinal material Dalbergia, pulverize it, add ethyl acetate and ethanol with a volume ratio of 1:1, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain extract I. Add 70% ethanol solution to the dregs, heat and reflux for 1 hour, extract twice, combine the filtrates to obtain extract II, concentrate the extract II into extract, dissolve the extract with 50% methanol solution, and use ODS-C 18 The column is separated, first use 5% methanol solution as mobile phase to obtain eluent I, then use 70% methanol solution as mobile phase to obtain eluent II, and obtain sample after concentration and drying; use preparative liquid chromatography The sample obtained by continuing to separate: the separation condition of preparative chromatography: the chromatographic column is a preparative column (ZORBAX Sb-C 18 ;21.2mm 250mm, 7 ), the mobile phase was water A and ac...

Embodiment 3

[0017] The analysis of the three effective components of balm

[0018] Chromatographic conditions: column Agilent Zorbax SB-C 18 column (4.6 mm × 150 mm , 5 μm); adopt gradient elution, mobile phase A phase is 0.2% glacial acetic acid aqueous solution, mobile phase B phase is the acetonitrile solution containing 0.2% glacial acetic acid; Gradient elution procedure is as follows: 0 minutes, mobile phase A is 0.2 % glacial acetic acid in water, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile; at 20 minutes, mobile phase A is 70% 0.2% glacial acetic acid in water, mobile phase B is 30% 0.2% glacial acetic acid in acetonitrile solution; at 30 minutes, mobile phase A was 50% 0.2% glacial acetic acid in water, and mobile phase B was 50% 0.2% glacial acetic acid in acetonitrile; at 35 minutes, mobile phase A was 50% 0.2% glacial acetic acid in water , Mobile phase B is 50% 0.2% glacial acetic acid in acetonitrile solution; flow rate 0.5mL / min; detection wavelen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an effective ingredient of rosewood heart wood. The effective ingredient mainly comprises isoliquiritigenin, medicarpin, dalbergin and 3'-methoxy violanone and is obtained by the following steps of: heating medicinal materials and extracting; concentrating, separating by using a silicagel column, eluting, concentrating eluent and drying; and continuously separating by using a liquid chromatogram, collecting solution, concentrating the collected solution and drying. The effective ingredient of the rosewood heart wood provided by the invention can be applied to the preparation of a medicament for treating and preventing cardiovascular disease. The method has a reasonable design and can be used for quickly and accurately obtaining the effective ingredient; and by the method, the quality of the medicament is easier to control during production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine extracts, and relates to a traditional Chinese medicine extract for treating cardiovascular diseases, in particular to a preparation method and application of an effective component extracted from Dalbergia basilica, the effective component mainly contains isoliquiritigenin, Several compounds such as di-dalterolin, dalterolin, and 3'-methoxydalterolin. Background technique [0002] Cardiovascular disease is the number one killer that endangers human health. In recent years, with the aging population of our country and changes in people's work, life, diet structure and environment, the incidence of coronary heart disease and other cardiovascular and cerebrovascular diseases has also increased year by year. Serious threaten the physical and mental health of the people. Many active substances in natural products have anti-myocardial ischemia and hypoxia effects, and some of them have been...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/48A61P9/00
Inventor 程翼宇王毅葛志伟李云飞
Owner ZHEJIANG UNIV