Method for preparing stichopus japonicus feed by using hydrolyzed fish meal and fermented bean pulp as main protein source

A technology for fermenting soybean meal and protein sources, which is applied in animal feed, animal feed, climate change adaptation, etc., can solve the problems of reduced feed utilization, heavy burden of feed digestion, and insufficient secretion of digestive enzymes, so as to save energy consumption and improve The survival rate and the effect of enhancing immune and disease resistance

Inactive Publication Date: 2011-07-27
DALIAN OCEAN UNIV
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Problems solved by technology

[0002] Due to the simple structure of the digestive system of sea cucumber, insufficient secretion of digestive enzymes, and weak ability to digest nutrients, the existing sea cucumber feed lacks the pretreatment process of raw materials, the digestibility of sea cucumber after ingestion is low, and the digestion burden of feed is too high. Heavy, not only affects the growth of sea cucumber, but also reduces the utilization rate of feed

Method used

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Embodiment Construction

[0013] The method for preparing sea cucumber feed with hydrolyzed fish meal and fermented soybean meal as the main protein source of the present invention, its steps are as follows:

[0014] a. Using high-quality fishmeal produced in Peru and high-quality soybean meal produced in Heilongjiang as basic raw materials and crushing them separately, the particle size after crushing is 200 mesh;

[0015] b. Soak the pulverized fish meal in deionized water, the volume ratio of fish meal and deionized water is 1:2; the soaking temperature is controlled at 25°C; and the pH of the mixture of fish meal and deionized water is adjusted to 2.0 with hydrochloric acid; then add to the stomach Protease and trypsin, the dosage ratio of pepsin and trypsin is 10:1, the mass ratio of pepsin and trypsin to fish meal and deionized water mixture is 1:2500; Hours of constant temperature hydrolysis; then the collected hydrolyzate is centrifuged to remove impurities, then absorbed by an organic carrier,...

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Abstract

The invention discloses a method for preparing stichopus japonicus feed by using hydrolyzed fish meal and fermented bean pulp as a main protein source, which comprises the following steps: grinding fish meal and bean pulp, which serve as the raw materials, to 200 meshes; soaking the fish meal in deionized water, regulating the pH value to 2.0 with hydrochloric acid, adding pepsase and trypsase, hydrolyzing fish meal for 20 hours at 37 DEG C, collecting hydrolysis solution, absorbing and drying to obtain hydrolyzed fish meal; sterilizing the bean pulp, placing the bean pulp into a fermentationtank, inoculating activated bacillus and lactobacillus, and fermenting at an initial temperature of 40 DEG C and pH value of 7 for 48 hours to obtain a fermented product; and mixing the hydrolyzed fish meal and the fermented bean pump to form the protein source of the feed, and mixing the protein source with an energy feed to obtain the stichopus japonicus feed. The method has the advantages of advanced process, high digestibility and capability of promoting growth. The feed prepared by the method can improve the immunity and disease resistance in stichopus japonicas and improve the culture survival rate of the stichopus japonicas.

Description

technical field [0001] The invention relates to a method for preparing sea cucumber feed, in particular to a method for preparing sea cucumber feed with hydrolyzed fish meal and fermented soybean meal as main protein sources. Background technique [0002] Due to the simple structure of the digestive system of sea cucumber, insufficient secretion of digestive enzymes, and weak ability to digest nutrients, the existing sea cucumber feed lacks the pretreatment process of raw materials, the digestibility of sea cucumber after ingestion is low, and the digestion burden of feed is too high. Heavy, not only affects the growth of sea cucumber, but also reduces the utilization rate of feed. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of high-quality and high-efficiency sea cucumber feed. The sea cucumber feed prepared by the method is conducive to improving digestibility, accelerating the growth speed of sea cucumber, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K10/12A23K10/22A23K50/80
CPCY02A40/818
Inventor 蒲红宇赵文许迪亮
Owner DALIAN OCEAN UNIV
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