Method for preparing stichopus japonicus feed by using hydrolyzed fish meal and fermented bean pulp as main protein source
A technology for fermenting soybean meal and protein sources, which is applied in animal feed, animal feed, climate change adaptation, etc., can solve the problems of reduced feed utilization, heavy burden of feed digestion, and insufficient secretion of digestive enzymes, so as to save energy consumption and improve The survival rate and the effect of enhancing immune and disease resistance
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[0013] The method for preparing sea cucumber feed with hydrolyzed fish meal and fermented soybean meal as the main protein source of the present invention, its steps are as follows:
[0014] a. Using high-quality fishmeal produced in Peru and high-quality soybean meal produced in Heilongjiang as basic raw materials and crushing them separately, the particle size after crushing is 200 mesh;
[0015] b. Soak the pulverized fish meal in deionized water, the volume ratio of fish meal and deionized water is 1:2; the soaking temperature is controlled at 25°C; and the pH of the mixture of fish meal and deionized water is adjusted to 2.0 with hydrochloric acid; then add to the stomach Protease and trypsin, the dosage ratio of pepsin and trypsin is 10:1, the mass ratio of pepsin and trypsin to fish meal and deionized water mixture is 1:2500; Hours of constant temperature hydrolysis; then the collected hydrolyzate is centrifuged to remove impurities, then absorbed by an organic carrier,...
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