Method for tissue culture of Acer fabri Hance by axillary buds
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A technology of tissue culture and axillary buds, which is applied in the field of tissue culture and reproduction of red-winged maple, to achieve the effect of solving the shortage of resources
Inactive Publication Date: 2011-08-03
NANJING LINJIANG GARDEN
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And about the research on the tissue culture of Acerus, there are few reports both at home and abroad.
In the past 5 years, there have only been reports on the use of tender stems (see Northern Horticulture, No. 9, 2010, Tissue Culture and Rapid Propagation Technology of Red-winged Acer) and young leaves (see Journal of Central South University of Forestry and Technology, 2010 No. Phase 4, the callus induction culture of the landscape tree species Red-winged Acer) is used as explants to cultivate the callus pathway, but there is no report on the in vitro rapid propagation of the axillary bud (organ) pathway of the red-winged Acer
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Embodiment 1
[0019] 1. Select 40 robust annual cuttings of the red-winged maple. Cut the young shoots of the year, remove the leaves, and use the stem segments as explants, each stem segment has an axillary bud, about 2cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaking table for 15 minutes. After rinsing with running water for 20 minutes, sterilize with 75% ethanol for 50 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water 4 times, shaking for 3 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium WPM+6-BA3.0mg / L+KT0.05mg / L+NAA0.05mg / L+GA 3 0.5mg / L. Each bottle is connected with 3 or 4 plants, and a total of 13 bottles are connected. Under the condition of temperature 24°C, light time 10h / day, and light intensity 2000lx, culture was carried out.
[0020] 2. S...
Embodiment 2
[0025] 1. Select 40 robust annual cuttings of the red-winged maple. Cut the young shoots of the year, remove the leaves, and use the stem segments as explants, each stem segment has an axillary bud, about 2cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaker for 20 minutes. After rinsing with running water for 30 minutes, sterilize with 75% ethanol for 60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water 4 times, shaking for 5 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium WPM+6-BA3.0mg / L+KT0.05mg / L+NAA0.05mg / L+GA 3 0.5mg / L. Each bottle is connected with 3 or 4 plants, and a total of 13 bottles are connected. Under the condition of temperature 26°C, light time 16h / day, and light intensity 1600lx, culture was carried out.
[0026] 2. Seven da...
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Abstract
The invention provides a method for tissue culture propagation of Acer fabri Hance by axillary buds, which comprises the following steps: disinfecting explants, inoculating the disinfected explants onto an induction medium for differentiation induction of buds, and normally culturing for 25 to 35 days under the conditions of a culture temperature between 20 and 26 DEG C, illumination time between 10 to 16 hours per day and an illumination intensity between 1,600 and 20,001x; cutting obtained tender shoots of Acer fabri Hance, and inoculating the cut tender shoots onto a subculture medium to perform subculture for 25 to 35 days; inoculating the sterile seedlings of the Acer fabri Hance into a rooting culture medium to perform rooting culture for 10 to 15 days under the conditions of a culture temperature between 20 and 26 DEG C, illumination time between 10 and 16 hours per day and illumination intensity between 1,600 and 20,001x; and transferring the obtained rotted sterile seedlings to a seedling bed to perform domestication culture for 30 to 60 days. The method can effectively solve the quick promotion problem of Acer fabri Hance.
Description
technical field [0001] The invention relates to a method for tissue culture and propagation of red-winged maple (Acer fabri Hance), and belongs to the technical field of seedling propagation of woody plants. Background technique [0002] The red-winged maple (Acer fabri Hance) belongs to the Acer family (Aceraceae). Also known as Acer Luofu (Guangxi Plant List), evergreen tree, 6-10 meters high; leaves leathery, lanceolate, 7-11 cm long, 2-3 cm wide, dark green above, light green below; samara when young Purple, yellowish brown or light brown when mature, nutlets protruding, about 5 mm in diameter, 8-10 mm wide in wings, 3-3.4 cm long together with the nutlets, opening in an obtuse angle, flowering period March-April, fruit period 5- September. It is mainly distributed naturally in Guangxi, Guangdong, Jiangxi, Hubei and other provinces in my country. Judging from the introduction and cultivation in Jiangsu, Zhejiang, Anhui, Shanghai and other places, it can withstand the ...
Claims
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