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Method for preparing liposome solution of chicken spleen transfer factor

A transfer factor and liposome technology, which is applied in the directions of liposome delivery, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of many clinical uses, unstable product quality, complicated preparation method and process, etc. Achieve good clinical effect, stable product performance, economical and practical effect

Inactive Publication Date: 2011-08-10
TIANJIN AGRICULTURE COLLEGE
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AI Technical Summary

Problems solved by technology

The existing broiler spleen transfer factor preparation method is complicated, the product quality is unstable, and the number of times of clinical use is high

Method used

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  • Method for preparing liposome solution of chicken spleen transfer factor
  • Method for preparing liposome solution of chicken spleen transfer factor

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Embodiment 1

[0029] See attached figure 1 .

[0030] The preparation method of broiler spleen transfer factor liposome solution comprises the following process steps:

[0031] 1. Preparation of High Concentration Chicken Transfer Factor

[0032] After the broiler spleen was washed with normal saline, the fat and capsule were removed with curved scissors. Mix the spleen and normal saline at a ratio of 2:1, homogenize it with a high-speed tissue masher at 10,000 rpm for 1 minute, put it into a plastic bottle, and freeze it in a -20°C refrigerator. Rinse with tap water to dissolve quickly. So repeated freezing and thawing 3 times. Put the frozen-thawed spleen tissue into a dialysis bag with a molecular cutoff of 10,000, seal it and put it into a white porcelain jar or a white porcelain plate, and put the same volume of normal saline containing 0.2% formaldehyde solution in the container at the same time, Dialyze with magnetic stirring in a 4°C refrigerator for 24-48hrs to obtain high-co...

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Abstract

The invention relates to a method for preparing a liposome solution of a chicken spleen transfer factor, belonging to the technical field of oral preparations. The method for preparing a liposome solution of the chicken spleen transfer factor comprises the following steps: 1, chicken transfer factor preparation process in which the spleen is cleaned, is crushed with water by a tissue triturator, and is frozen and thawed repeatedly for 3 to 8 times, the spleen tissue which is subjected to freezing and thawing treatment is filled in an analysis bag, and the sealed bag is put in a vessel filled with normal saline of a formaldehyde solution, and is stirred and analyzed by magnetic force to obtain the spleen transfer factor of the chicken; 2, liposome preparation process in which lecithin and cholesterol are put in an evaporative flask, vitamin E and chloroform are added, and the mixture is dissolved, decompressed, rotated and evaporated to form a liposome in a rotary flask; and 3, transfer factor liposome preparation process in which the chicken transfer factor is mixed with the liposome, and is homogenized with a homogenizer to prepare the nanometer chicken spleen transfer factor liposome. The method has the advantages of simple process, economy and practicality, stable performance, good clinical effect, wide application range and the like.

Description

technical field [0001] The invention belongs to the technical field of oral preparations, in particular to a preparation method of broiler spleen transfer factor liposome solution. Background technique [0002] At present, chicken transfer factor (Chicken Transfer Factor, referred to as CTF) is a kind of protein-free soluble and dialyzable protein composed of oligonucleotides and polypeptides, which is processed and prepared from broiler spleen as raw material. , Small molecular substances that can be ultrafiltered. The monitoring results of lymphocyte transfer test and serological test show that: chicken transfer factor can significantly improve the level of cellular immunity (t>2.878, P<0.01=, significantly improve the level of humoral immunity (t>2.101, P<0.05=; use CTF alone The injection has obvious therapeutic effects on Newcastle disease, bursal disease and Marek's disease, can reduce the mortality rate of chickens with Marek's disease by 27.5-40%, and re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/127A61K9/08A61P37/04A61P31/14A61P31/22A61K35/26
Inventor 李富桂张文生王志龙常蕊
Owner TIANJIN AGRICULTURE COLLEGE
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