Method for quickly propagating high-quality liquorice seedlings

Abstract

The invention relates to a method for quickly propagating high-quality liquorice seedlings. The method is realized by sequentially comprising the following steps of: firstly, washing seeds with running water, sterilizing the seeds with 75% alcohol and washing 3-4 times with sterile water; then sterilizing the seeds with 0.1% corrosive sublimate, and washing 3-4 times with sterile water; then inoculating the seeds to an MS (Murashige and Skoog) culture medium for culturing aseptic seedlings for 1 month; then cutting stalks of the aseptic seedlings; chopping the stalks into stalk sections with the length of 0.5cm; and inoculating into a 0.5mg / L MS+KH2PO310mg / L+NAA culture medium to be cultured continuously. The method for quickly propagating the high quality liquorice seedlings has the advantages that the rooting rate of the aseptic seedlings reaches 100%, aseptic liquorice nursery stocks can be quickly propagated, the cycle is short, and the method is not influenced by factors, such as seasons, weather, natural disaster and the like and can realize industrial production. By adopting the technology, many problems of low propagation capability, shortage of resources, pesticide residue and the like of a medicinal plant can be solved. Since the technology for quickly propagating aseptic liquorice seedling stalks is firstly utilized in Gansu Province, in China, novel liquorice germ plasms in Gansu can be quickly propagated and meet market requirements and have strategic meanings in the aspects of preventing wind, stabilizing sands, protecting ecological environment and germ plasm.

Description

technical field [0001] The invention relates to a method for cultivating licorice, in particular to a method for rapid propagation of high-quality licorice seedlings. Background technique [0002] Licorice is one of the five authentic medicinal materials in Gansu Province. It is mainly distributed in the Hexi area. It is a wild sand-fixing plant under national key protection. It plays an important role in protecting the ecological environment and grassland resources and preventing land desertification. In recent years, due to the sharp increase in the use of licorice in the pharmaceutical, food and cosmetic industries, people have destructively excavated wild licorice, making wild plants almost extinct and seriously destroying the ecological environment. Although our province has made some achievements in the artificial cultivation of licorice, it usually takes 4 years for the artificial cultivation of licorice to produce seeds, while the harvest of licorice generally only t...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to solve the problem of licorice variety breeding and improved seed propagation in our province, to solve the problem of long seed propagation cycle, to provide a method for rapid propagation of high-quality licorice seedlings, to obtain high-quality seedlings, and to shorten the propagation time of licorice. Cycle, speed up the breeding process of improved licorice varieties, carry out large-scale chemical plant production, meet market demand for licorice, protect wild licorice natural resources and a good natural ecological environment