Method for quickly propagating high-quality liquorice seedlings

A high-quality, licorice technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of long seed reproduction cycle, achieve the effects of short cycle, ecological environment protection, and germplasm protection

Inactive Publication Date: 2011-08-17
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to solve the problem of licorice variety breeding and improved seed propagation in our province, to solve the problem of long seed propagation cycle, to provide a method for rapid propagation of high-quality licorice seedlings, to obtai

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The present invention has the following sequential steps:

[0013] a. First, select high-quality licorice seeds and wash them with running water for 2 hours.

[0014] b. Disinfect the washed seeds with 75% alcohol for 1 min, and then rinse the alcohol-sterilized seeds with sterile water for 3 to 4 times.

[0015] c. The seeds rinsed with sterile water are then sterilized with 0.1% mercuric acid for 7 minutes, and then the mercury-sterilized seeds are rinsed with sterile water for 3 to 4 times.

[0016] d. Finally, inoculate the sterilized seeds in MS medium to cultivate sterile seedlings. The cultivation temperature is 26° C., the light intensity is 1500 LX, and the light time is 18 h / d.

[0017] e. After cultivating for 1 month, cut off the stems of the sterile seedlings, cut them into 0.5cm long and short stems, and insert them into MS+KH 2 PO 4 10mg / L+NAA 0.5mg / L medium to continue the rapid expansion and cultivation of sterile vaccines.

Embodiment 2

[0019] The method of embodiment 2 is the same as that of embodiment 1, except that the culture temperature of the sterile seedlings in step d is 27° C., the light intensity is 1800 LX, and the light time is 20 h / d.

Embodiment 3

[0021] The method of embodiment 3 is the same as that of embodiment 1, except that the culture temperature of the sterile seedlings in step d is 28° C., the light intensity is 2000 LX, and the light time is 24 h / d.

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Abstract

The invention relates to a method for quickly propagating high-quality liquorice seedlings. The method is realized by sequentially comprising the following steps of: firstly, washing seeds with running water, sterilizing the seeds with 75% alcohol and washing 3-4 times with sterile water; then sterilizing the seeds with 0.1% corrosive sublimate, and washing 3-4 times with sterile water; then inoculating the seeds to an MS (Murashige and Skoog) culture medium for culturing aseptic seedlings for 1 month; then cutting stalks of the aseptic seedlings; chopping the stalks into stalk sections with the length of 0.5cm; and inoculating into a 0.5mg/L MS+KH2PO310mg/L+NAA culture medium to be cultured continuously. The method for quickly propagating the high quality liquorice seedlings has the advantages that the rooting rate of the aseptic seedlings reaches 100%, aseptic liquorice nursery stocks can be quickly propagated, the cycle is short, and the method is not influenced by factors, such as seasons, weather, natural disaster and the like and can realize industrial production. By adopting the technology, many problems of low propagation capability, shortage of resources, pesticide residue and the like of a medicinal plant can be solved. Since the technology for quickly propagating aseptic liquorice seedling stalks is firstly utilized in Gansu Province, in China, novel liquorice germ plasms in Gansu can be quickly propagated and meet market requirements and have strategic meanings in the aspects of preventing wind, stabilizing sands, protecting ecological environment and germ plasm.

Description

technical field [0001] The invention relates to a method for cultivating licorice, in particular to a method for rapid propagation of high-quality licorice seedlings. Background technique [0002] Licorice is one of the five authentic medicinal materials in Gansu Province. It is mainly distributed in the Hexi area. It is a wild sand-fixing plant under national key protection. It plays an important role in protecting the ecological environment and grassland resources and preventing land desertification. In recent years, due to the sharp increase in the use of licorice in the pharmaceutical, food and cosmetic industries, people have destructively excavated wild licorice, making wild plants almost extinct and seriously destroying the ecological environment. Although our province has made some achievements in the artificial cultivation of licorice, it usually takes 4 years for the artificial cultivation of licorice to produce seeds, while the harvest of licorice generally only t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 蔺海明柳福智
Owner GANSU AGRI UNIV
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