Method for preparing ginsenoside Rh2

A technology of ginsenosides and total ginsenosides, which is applied in the field of microorganisms and can solve problems such as low yield

Active Publication Date: 2011-08-17
长白山皇封参业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the object of the present invention aims at the low yield of the existing ginseno

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 5 mL of activated Lactobacillus delbrueckii subsp. bulgaricus (L.b-01) and inoculate 1 L of MRS medium, add 10 g of total ginsenosides, ferment at 37-39°C for 240-248 hours, collect the fermentation broth, and filter. Then add 0.5g β-glucosidase, react at 88°C for 240h, collect the reaction solution after the reaction, and filter. The filtrate was adsorbed with macroporous resin AB-8, and eluted with 600 mL of 70% ethanol. The eluate was extracted with ethanol with a volume fraction of 95%, the extract was filtered to collect the filtrate, and dried to obtain 24.32 g of ginsenoside Rh with a conversion rate of 43.2%.

Embodiment 2

[0027] Take 20mL of activated Lactobacillus delbrueckii subsp. bulgaricus (L.b-3) and inoculate 1L of MRS medium, add 15g of total ginsenosides, ferment at 37-39°C for 240-248h, collect the fermentation broth, and filter. Then add 0.2g α-arabinosidase, react at 92°C for 360h, collect the reaction solution after the reaction, and filter. The filtrate was adsorbed with macroporous resin AB-8, and eluted with 500 mL of 70% ethanol. The eluate was extracted with ethanol with a volume fraction of 95%, the extract was filtered to collect the filtrate, and dried to obtain ginsenoside Rh2 with a conversion rate of 45.6%. Purified by silica gel column chromatography and dried to obtain 4.08 g of pure ginsenoside Rh2, the purity of which was 96.4% by HPLC.

Embodiment 3

[0029] Take 15 mL of activated Lactobacillus delbrueckii subsp. bulgaricus (L.b-MYC) and inoculate 1 L of MRS medium, add 20 g of total ginsenosides, ferment at 37-39°C for 240-248 hours, collect the fermentation broth, and filter. Then add 0.3g α-rhamnosidase, react at 90°C for 280h, collect the reaction solution after the reaction, and filter. The filtrate was adsorbed with macroporous resin AB-8, and eluted with 500 mL of 70% ethanol. The eluate was extracted with ethanol with a volume fraction of 95%, the extract was filtered to collect the filtrate, and dried to obtain ginsenoside Rh2 with a conversion rate of 43.5%. Purified by silica gel column chromatography and dried to obtain 4.12 g of pure ginsenoside Rh2, the purity of which was 97.2% by HPLC.

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Abstract

The invention relates to the field of microorganism, in particular to a method for preparing ginsenoside Rh2. The method comprises the following steps: taking activated lactobacillus delbrueckii subsp bulgaricus to inoculate MRS (magnetic resonance spectrum) culture medium, adding total ginsenoside; fermenting for 240-248 hours at 37-39 DEG C; collecting fermentation liquor and saponin-glycosidases for reacting at 240-360 hours at 88-92 DEG C; collecting reaction solution for filtration, absorbing the filtrate by using microporous resin and then eluting the absorbed filtrate to by 70 percent ethanol; extracting with 95 percent ethanol; and then filtering, and drying the filtrate to obtain the ginsenoside Rh2. The preparation method is simple to operate, mild for reaction conditions, utilizes the lactobacillus delbrueckii subsp bulgaricus to conduct biotransformation on the total ginsenoside, and saponin-glycosidases to prepare the ginsenoside Rh2, thus being high in transformation rate, being used for preparing a large amount of ginsenoside Rh2, and further realizing the industrialization of the ginsenoside Rh2.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a preparation method of ginsenoside Rh2. Background technique [0002] Ginseng is the root of the Araliaceae plant. It is known as the "King of Herbs". It is a precious medicinal material that is well-known both at home and abroad. Studies have found that ginseng mainly contains saponins, peptides, amino acids, vitamins and other substances, among which ginsenoside (Ginsenoside) is the main component of ginseng efficacy, which widely acts on the nervous system and immune system of the human body. The treatment shows a distinctive and unique effect. There are more than 40 kinds of ginsenoside monomers, such as Rb1, Rb2, Rb3, Rc, Rd, Rg1, Rg2, Rg3, Rh1, Rh2 and Re, etc. [0003] In recent years, a large number of studies have found that under the action of biological enzymes, ginsenosides in the body are metabolized into Rg3, Rg5, Rh2, Rh3, C-K, protopanaxadiol, Rg2 with less sugar ...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12R1/225
Inventor 佟心陈凯千
Owner 长白山皇封参业股份有限公司
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