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Method for detecting release situation of nicotine in buccal tobacco products

A technology of tobacco products and detection port, which is applied in the field of tobacco, can solve the problems of undetectable nicotine release rate, instability of supporting liquid film device, large fluctuation of measurement results, etc., and achieves the advantages of easy promotion, short detection period and high sensitivity Effect

Active Publication Date: 2012-06-27
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method realizes the online detection of the total release of nicotine, but the supporting liquid film device is unstable, the liquid film is easy to lose, the measurement results fluctuate greatly, and the release rate of nicotine cannot be detected

Method used

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  • Method for detecting release situation of nicotine in buccal tobacco products
  • Method for detecting release situation of nicotine in buccal tobacco products
  • Method for detecting release situation of nicotine in buccal tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The flow rate was 0.2 mL / min, the temperature was 37°C, and the pH of the artificial saliva was 6.7; the detection of nicotine in the released liquid was performed by high-performance liquid chromatography, and the chromatographic parameters were as follows: the chromatographic column was Waters XTerra RP C18 reversed-phase chromatographic column, The mobile phase is methanol and 20 mmol of phosphate buffer solution (triethylamine is added in the mobile phase, and the content is 0.2%, the purpose is that triethylamine can effectively improve the tailing of the nicotine chromatographic peak, the retention time becomes larger, and the phosphoric acid pH of salt buffer solution = 6.0), the ratio is 23:77, the flow rate is 1 mL / min, and the column temperature is 35 °C.

[0036] Add artificial saliva with a pH of 6.7 into the solvent storage bottle, turn on the constant temperature tank and constant flow pump, set the flow rate to 0.2 mL / min, and after the system stabilizes t...

Embodiment 2

[0038] The flow rate was 0.4mL / min, the temperature was 37°C, and the pH of the artificial saliva was 6.4; the detection of nicotine in the release liquid was performed by high performance liquid chromatography, and the chromatographic parameters were the same as in Example 1.

[0039] Add the artificial saliva with a pH of 6.4 into the solvent storage bottle, turn on the constant temperature tank and constant flow pump, and set the flow rate to 0.4 mL / min. After the system stabilizes to 37 °C, open the glass ground plug and place the sample flat Cover the stand with a ground stopper, and at the same time, collect once every 5 minutes for a total of 60 minutes. After the release solution is collected, take out the remaining sample after release and put it into a 50 mL Erlenmeyer flask, add 20 mL of ethanol and A mixed solution of 5% NaOH solution (9:1), ultrasonically extracted for 30 min. After the nicotine release solution and the residual sample extraction solution pass thr...

Embodiment 3

[0041] The flow rate was 1 mL / min, the temperature was 37 °C, and the pH of the artificial saliva was 7.0; the detection of nicotine in its release liquid was performed by high-performance liquid chromatography, and the chromatographic parameters were the same as in Example 1

[0042] Add artificial saliva with a pH of 7.0 to the solvent storage bottle, turn on the constant temperature tank and constant flow pump, set the flow rate to 1 mL / min, and after the system stabilizes to 37 °C, open the glass ground plug and place the sample flat On the sample holder, cover the ground plug, and time it at the same time, respectively at 1min, 2min, 3min, 4min, 5min, 6min, 7min, 8min, 9min, 10min, 12min, 14min, 16min, 18min, 20min, 24min, 28min, At 32 minutes, the released liquid was collected into corresponding collection bottles. After the release solution was collected, the residual sample after release was taken out and placed in a 50 mL Erlenmeyer flask, and 20 mL of a mixed solutio...

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Abstract

The invention relates to a method for detecting a release situation of nicotine in buccal tobacco products, which is characterized by comprising the steps of simulating the release situation of the nicotine in the buccal tobacco products in the human mouth, instantly quantifying the release quantity and the release speed and then detecting the release speed and the release degree of the nicotine in the buccal tobacco products. The release and the collection of the nicotine are realized through a release and collection device in vitro; by means of the device, the release process of the nicotine in the buccal tobacco products in the human mouth can be simulated and nicotine release solutions can be the instantly collected, wherein the release process is a process that the nicotine is continuously extracted from the buccal tobacco products by utilizing artificial saliva at a constant temperature; and the detection of the nicotine content in the release solution is realized through a highperformance liquid chromatography. The method has the advantages: the release speed and the release degree of the nicotine in various buccal tobacco products can be rapidly and accurately detected with good repeatability, high sensitivity and short detection period and is suitable for the rapid analysis of mass samples for different industries and different purposes.

Description

technical field [0001] The present invention relates to the field of tobacco, in particular to a method for detecting the release of nicotine from buccal tobacco products, through which specific data support can be provided for researching or measuring the safety and quality control of buccal tobacco products . Background technique [0002] Bouched tobacco products are tobacco products that crush tobacco leaves and pack tobacco powders of different particle sizes into pouches after steam heat treatment and other processes. The biggest difference between this type of product and cigarettes is that it is a smokeless tobacco product. When using it, it is placed between the upper lip of the mouth and the gums. The tobacco components are dissolved in saliva and absorbed through the oral mucosa. [0003] In recent years, due to the restrictions on smoking in public places by governments of various countries, the consumption of buccal tobacco products has been increasing year by y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
Inventor 谢剑平孟全新张杰宗永立李鹏
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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