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Method for extracting active ingredients from honeysuckle leaves

A technology for active ingredients and honeysuckle leaves, which is applied in the field of extracting active ingredients from honeysuckle leaves, can solve the problems of insufficient utilization of honeysuckle, large consumption, waste of resources, etc., and achieves expanded application value, high organic acid content, and improved recovery rate. Effect

Inactive Publication Date: 2013-07-03
HEBEI KEXING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the extraction of active ingredients and effective parts of honeysuckle only uses honeysuckle flower buds as raw materials, and methanol, ether or ethyl acetate as extraction solvents, mainly including direct decoction, dipping, heat reflux, etc. Moreover, various extraction methods are still traditional water extraction and alcohol precipitation or alcohol extraction and water precipitation, which will produce a large amount of precipitation in the subsequent treatment, which will not only cause a large loss of active ingredients such as chlorogenic acid, but also bring greater difficulties to the process operations such as filtration , time-consuming and energy-consuming
In addition, these methods all have many disadvantages such as long extraction period, toxic solvents used, large dosage, high temperature required to cause degradation of active components, etc., and the final extraction rate and recovery rate of the target product are very low
Therefore, the traditional processing technology can not make full use of the active ingredients in honeysuckle, thereby greatly reducing its application value and causing a lot of waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. Pretreatment of fresh honeysuckle leaves: After drying 500g of fresh honeysuckle leaves at 45°C for 14 hours, crush them into 100-mesh powder, refrigerate at 5°C, and set aside;

[0023] b. Supercritical CO2 extraction:

[0024] Put 200g of the powder obtained in step a into the extraction kettle, the extraction temperature is 35°C, the extraction pressure is 55 MPa, and the CO2 flow rate is 15 kg / h; the pressure of the primary separation kettle is 15 MPa, the temperature is 35°C, and the pressure of the secondary separation kettle is 5 MPa, the temperature is 50°C, collect the product and set aside;

[0025] c. Ultrasonic enhanced extraction: take 100g of the product obtained in step b, add 1000g of absolute ethanol, the temperature is 20°C, the ultrasonic power is 200w, ultrasonic extraction is 30 min; Centrifuge at a speed of 20 min;

[0026] Then add 500 g of absolute ethanol to the precipitated part, repeat the above extraction steps; combine the supernatant o...

Embodiment 2

[0030] a. Pretreatment of fresh honeysuckle leaves: After drying 500g of fresh honeysuckle leaves at 60°C for 12 hours, crush them into 40-mesh powder, refrigerate at 10°C, and set aside;

[0031] b. Supercritical CO2 extraction:

[0032] Put 200g of the powder obtained in step a into the extraction kettle, the extraction temperature is 25°C, the extraction pressure is 20 MPa, and the CO2 flow rate is 10 kg / h; the pressure of the primary separation kettle is 18 MPa, the temperature is 25°C, and the pressure of the secondary separation kettle is 8MPa, the temperature is 25°C, collect the product and set it aside;

[0033] c. Ultrasonic enhanced extraction: Take 100g of the product obtained in step b, add 300g of absolute ethanol, the temperature is 50°C, the ultrasonic power is 120w, and the ultrasonic extraction is 60min; Centrifuge for 10 min;

[0034] Then add 300g of absolute ethanol to the precipitated part, repeat the above extraction steps; combine the supernatant obta...

Embodiment 3

[0038] a. Pretreatment of fresh honeysuckle leaves: After drying 500g of fresh honeysuckle leaves at 50°C for 17 hours, crush them into 300-mesh powder, refrigerate at -20°C, and set aside;

[0039] b. Supercritical CO2 extraction:

[0040] Put 200g of the powder obtained in step a into the extraction kettle, the extraction temperature is 30°C, the extraction pressure is 60MPa, the CO2 flow rate is 25kg / h; the pressure of the primary separation kettle is 10MPa, the temperature is 30°C, the pressure of the secondary separation kettle is 6MPa, the temperature at 40°C, collect the product and set aside;

[0041] c. Ultrasonic enhanced extraction: take 100g of the product obtained in step b, add 600g of acetone, the temperature is 40°C, the ultrasonic power is 250w, and ultrasonic extraction is performed for 40min; the extract is centrifuged at 0°C at a speed of 5000 r / min for 15min;

[0042] Then add 400g of acetone to the precipitation part, repeat the above extraction steps; c...

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PUM

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Abstract

The invention discloses a method for extracting active ingredients from honeysuckle leaves, which comprises the following steps: pretreating fresh honeysuckle leaves; carrying out supercritical CO2 extraction; strengthening and leaching with ultrasonic waves; and concentrating the supernatant liquid to finally obtain the active ingredients. By using the method disclosed by the invention for extracting the active ingredients from the honeysuckle leaves, the content of organic acids can reach more than 50%, thereby greatly improving the application value; and the obtained products can be applied to many occasions such as pharmacy, health products and the like.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from leaves of Chinese medicinal materials, in particular to a method for extracting active ingredients from leaves of honeysuckle. Background technique [0002] Honeysuckle is a commonly used Chinese herbal medicine. Its main active ingredients are chlorogenic acid and isochlorogenic acid. Other active ingredients include flavonoids and volatile oils. It has antibacterial, antiviral, antipyretic, anti-inflammatory, liver-protecting, and hemostatic properties. , anti-oxidation, immune regulation and other effects. Thousands of years of traditional experience, modern pharmacological experiments and clinical application have proved that honeysuckle has strong antibacterial effect and good therapeutic effect on various germs. [0003] In the prior art, the extraction of active ingredients and effective parts of honeysuckle only uses honeysuckle flower buds as raw materials, and methanol,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/355A61P31/04A61K127/00
Inventor 赵素娟牛志勇陈淑芳王志香王欢郭艳玉田会彦
Owner HEBEI KEXING PHARMA