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Active dry yeast protectant and application thereof

A technology of active dry yeast and protective agent, which is applied in the direction of preservation of microorganisms, microorganisms, biochemical equipment and methods, etc. It can solve the problems of increased activity loss, increased product moisture, and slow drying speed, and achieves improved fermentation power and preservation rate. Improved effect

Active Publication Date: 2011-08-31
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different yeast strains have certain differences
[0008] 2. The effect of moisture on the activity of dry yeast
[0011] 3. The effect of packaging method on the activity of dry yeast
[0016] If the drying temperature is too high, it is easy to inactivate and kill the cells. If the temperature is too low, the drying time will be prolonged and the moisture content of the product will increase.
The drying speed must also be controlled within a certain range. If the drying speed is too slow, the drying time will be prolonged, and the activity loss of yeast cells due to oxidation will increase during the drying process; if the drying speed is too fast, the yeast cells will easily cause cell membrane damage during the rapid dehydration process. damage, cell death

Method used

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  • Active dry yeast protectant and application thereof
  • Active dry yeast protectant and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0053] Preparation of Yeast Fermentation Broth

[0054] Sterilize the real tank in a 50L fermenter with 25L of fermentation basal medium, and the sterilization condition is 120°C for 30min. Then, when the temperature was lowered to 32°C, 3L of seed culture solution after inoculation and cultivation was inserted, the pH was adjusted to 5.2, the culture temperature was controlled at 30°C, sugar solution and nutrient salt solution were added, and the total fermentation time was controlled to be 21 hours. The addition was completed 5 hours before the end, and the amount of the sugar solution was added according to the growth of the yeast, so that the glucose content in the fermentation system was always controlled below 0.5%. During the fermentation process, the pH is controlled at 5.2, and the aeration rate is 1.2-2.5vvm to obtain a yeast fermentation culture liquid with a cell concentration of 4.5-5.5%.

[0055] Culture temperature control: the culture temperature is 30°C when ...

Embodiment 1

[0061] An active dry yeast protectant, the components are as follows, all in weight percentage:

[0062] Glyceryl monostearate 40%, sorbitan monostearate 30%, sorbitan monooleate 24%, glycerin 6%. The above ingredients were prepared into a 20% emulsion with 90°C distilled water. Use after cooling down to 45°C.

[0063] Add the above-mentioned active dry yeast protective agent to the yeast milk, accounting for 2.5% by weight of the dry matter in the yeast milk, stir thoroughly for 40 minutes, then filter and collect the yeast mud, with a water content of 28-30%, extrude and granulate to a diameter of 0.1mm The columnar particles are dried in air at 45°C to a moisture content of 5.0-5.5%. Measure active dry yeast living cell rate respectively: 85%; Active dry yeast preservation rate: 87%; Active dry yeast fermentability (CO 2 ) is 530mL / h, and its scanning electron microscope picture is as follows figure 1 shown.

Embodiment 2

[0067] Active dry yeast protective agent as described in embodiment 1, difference is:

[0068] Glyceryl monostearate 44%, sorbitan monostearate 25%, sorbitan monooleate 26%, glycerin 5%.

[0069] The live cell rate of active dry yeast was determined to be 87% respectively; the preservation rate of active dry yeast was 89%; the fermentability of active dry yeast (CO 2 ) is 530mL / h.

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Abstract

The invention relates to an active dry yeast dehydration protectant and application thereof and belongs to the technical field of microorganism products and food additives. The active dry yeast protectant comprises the following components: glyceryl monostearate, sorbitan monostearate, sorbitan monooleate (Span80) and glycerin. The active dry yeast protectant can obviously increase the living cell rate of active dry yeast to 86 to 90 percent, obviously increase the preserving rate of active dry yeast to 88 to 90 percent and obviously increase the fermentation ability of active dry yeast to 530 to 550mL / h.

Description

technical field [0001] The invention relates to an active dry yeast dehydration protectant and application thereof, belonging to the technical field of microbial products and food additives. Background technique [0002] Active dry yeast is made from fresh yeast or squeezed yeast, which is granulated, dried and dehydrated. The activity of dry yeast is usually expressed by fermentation power. [0003] Under normal circumstances, 3.5g of fresh yeast is required to produce 1g of active dry yeast, but in terms of activity (fermentation power), 1g of active dry yeast is only equivalent to 2-3g of fresh yeast: after rehydration, the test of viable cells is generally 80- 90%. Fresh yeast is made into active dry yeast, and after a certain period of storage and rehydration before use, there is a certain loss of activity. [0004] Factors affecting the viability of dry yeast are as follows: [0005] 1. The effect of chemical composition on the activity of dry yeast [0006] The co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/865
Inventor 王瑞明王腾飞马春玲李丕武
Owner QILU UNIV OF TECH
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