Breeding method of easy-dry type self-incompatible line of Chinese cabbage anther

Abstract

The invention discloses a breeding method of an easy-dry type self-incompatible line of Chinese cabbage anther. The method comprises the following steps: (1) selecting the easy-dry type self-incompatible line of anther as breeding protospecies to plant; (2) collecting the anther of the self-incompatible line blossoming in the day and not releasing pollen at 8-10 a.m.; (3) processing the anther so that the anther is loosened and releases pollen until the pollen can be dipped; (4); manually opening the bud to blossom in 3-5 days on the self-incompatible line, and exposing the stigma; (5) manually dipping the pollen obtained in the step (3) with a finger or a rubber, and smearing the pollen on the stigma obtained in the step (4); and (6) collecting the self-pollinated seeds after hulling. As easy-dry type self-incompatible line of Chinese cabbage anther has short pollen releasing time and small pollen quantity, the method disclosed by the invention adopts a mode of in vitro pollen collection and artificial pollination, improves the pollen quantity, overcomes the self-incompatibility of Chinese cabbage, can increase the seed collection quantity by 100-200 times, and realizes high breeding efficiency and yield.

Description

technical field [0001] The invention relates to a breeding method of Chinese cabbage, in particular to a breeding method of a Chinese cabbage anther easy-to-dry type self-incompatibility line. Background technique [0002] At present, several seed production methods adopted in the utilization of heterosis in Chinese cabbage at home and abroad include: "self-incompatibility lines", "advanced inbred lines" and "male sterility". Among them, "self-incompatibility" does not require complicated procedures such as the transfer of male sterile lines, and can be directly used for hybrid seed production after purification. It is convenient, fast, and easy to obtain high-quality seeds. It has become the current domestic "Male sterile line" has been basically eliminated due to its limitations; Easy to control, easy to obtain high-quality hybrids, low cost and other characteristics are developing rapidly, especially "cytoplasmic sterility" and "cytoplasmic-nuclear interaction male steri...

AI Technical Summary

Problems solved by technology

In terms of self-incompatibility breeding, at present, Japan, South Korea, etc. mainly use 4% carbon dioxide gas treatment under airtight conditions every 7 days during the flowering period, combined with bee pollination to overcome self-incompatibility to breed the original species. Although the method breeds a large number of seeds, it requires the entire breeding group to be under airtight conditions, a carbon dioxide detector, liquid carbon dioxide, and precise concentration of carbon dioxide treatment are required, making it difficult to promote in my country

In China, 2-3% salt water is sprayed at the flowering stage, combined with bees or artificial pollination to overcome self-incompatibility ("Progress in Disease-resistant Breeding of Main Vegetables in China"). The method is simple and easy to operate. It is prevalent in my country; this method has a good effect on most self-compatible lines and self-incompatible lines; but it has little effect on some self-incompatible lines; another method is to manually peel the buds, and then Manually use rubber or hands to directly use the pollen of the plant (line) to pollinate the peeled flower buds to overcome the incompatibility. The self-incompatibility of the self-incompatibility line that is easy to shrivel, pollen is not easy to disperse, and the pollen time is very short, due to the serious shortage of pollen, the seed collection is extremely low, and it is also difficult to overcome its self-incompatibility

The above method can breed more original species for most incompatible lines; but for some highly incompatible lines spraying salt water combined with releasing bees, usually 10-200 grams of original species are bred per 667 square meters, less than The 5% of conventional self-incompatible lines is mainly because when these self-incompatible lines encounter salt water or atmospheric high temperature and drought, the anther loose pollen time is very short, even if the pollen is loose, the pollen quantity is small, and the pollen quantity is insufficient due to early drying pollination, and at the same time it is a self-incompatible line, direct pollination at the flowering stage is not fruitful