Bionic three-dimensional fluid shear stress cell culture device and shear stress loading method thereof

Abstract

The invention discloses a bionic three-dimensional fluid shear stress cell culture device and a shear stress loading method thereof. The bionic three-dimensional fluid shear stress cell culture device is characterized by consisting of a peristaltic pump (1), a perfusion small chamber (2), a 1-to-8 switcher (3), an air filter membrane (4), a waste liquid bottle (5), a straight-through valve (6), aculture medium (7), a liquid storage bottle (8) and a processing silicon tube (9), wherein one end of the perfusion small chamber is connected with the peristaltic pump (1), and the other end of the perfusion small chamber is connected with the waste liquid bottle (5) through the 1-to-8 switcher (3); the waste liquid bottle is connected with the liquid storage bottle (8) through the straight-through valve (6); and the liquid storage bottle (8) is connected with the peristaltic pump (1) through the 1-to-8 switcher (3).

Description

technical field [0001] The invention relates to a bionic three-dimensional fluid shear stress cell culture device and a shear stress loading method thereof, which are used for studying the responses of various cells to controllable fluid shear stress in a three-dimensional culture state, and belong to the field of biomechanics. Background technique [0002] The extracellular matrix composed of inorganic and organic components constitutes a complex and highly organic bone tissue. Appropriate mechanical force loading plays an important role in bone, which can promote bone formation and reduce bone resorption. Conversely, the lack of appropriate stimulation will Affects the production of bone matrix protein and mineralized substances, reduces bone formation, and increases bone resorption. Therefore, many in vitro experiments have begun to study the changes in the response of bone cells to different mechanical stimuli (including fluid shear stress, hydrostatic pressure, and stre...

AI Technical Summary

Problems solved by technology

[0004] However, the few self-made three-dimensional fluid shear stress cell culture systems currently used have disadvantages such as complex assembly, non-perfusion flow path bypass, and poor observability.

For example: the culture system designed by Xu Shanglong et al. (Progress in Biochemistry and Biophysics, 2006, 33 (9): 895-901) is to directly immerse the scaffold material in the culture bottle, and a large amount of non-toxic substances are produced when the culture medium flows through the culture bottle. The perfusion path cannot ensure that the fluid flows vertically through the entire scaffold, and the cells on the surface and inside of the scaffold cannot receive uniform mechanical stimulation

In the culture system designed by Li Xiang et al. (Acta Bioengineering, 2005, 21(4): 579-583), the scaffold material is embedded in the silicone rubber sealing ring, which is difficult to assemble and easily causes pollution.

The culture system designed by Wu Dan et al. (Journal of Clinical Stomatology, 2007, 23 (4): 197-199) only uses one perfusion chamber. If you want to increase the sample size, you need to repeat the experiment many times, which increases the number of operations. The design of the perfusion chamber is opaque, and the liquid flow and cell culture in the chamber cannot be observed, which brings inconvenience to the experiment

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