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Method for preparing fermentation carbon source by hydrolysis and acidification of biomass raw materials

A biomass raw material, hydrolysis and acidification technology, applied in the direction of fermentation, etc., can solve the problems of low hydrolysis efficiency of lignocellulose, increase the production cost of products, etc., and achieve the effect of saving consumption, increasing concentration, and cheap fermentation carbon source

Inactive Publication Date: 2011-09-07
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
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  • Description
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AI Technical Summary

Problems solved by technology

However, only relying on the enzymes carried by the microorganisms itself, the hydrolysis efficiency of lignocellulose is very low, and the addition of cellulase will greatly increase the production cost of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) The corn stalks are pretreated by steam explosion, dried and crushed to 20 mesh;

[0027] 2) Using the domesticated activated sludge as the seed sludge, mix the steam explosion pretreated corn stalks evenly with the mass ratio of 1:1 to the activated sludge, then add water to adjust the solid-liquid ratio to 1:10, and adjust the pH to After 5.5, nitrogen gas was introduced into the reaction system, and anaerobic acidification was carried out at 35°C for 5 days.

[0028] 3) Solid-liquid separation is carried out after acidification, and the acidified liquid obtained by acidification is used as a fermentation carbon source of PHA.

Embodiment 2

[0030] 1) The corn stalks are pretreated with dilute acid, dried and crushed to 40 mesh;

[0031] 2) Using the domesticated activated sludge as the seed sludge, mix the dilute acid pretreated corn stalks evenly with the mass ratio of 1:1 to the activated sludge, then add water to adjust the solid-liquid ratio to 1:5, and adjust the pH to After 6.0, nitrogen gas was introduced into the reaction system, and anaerobic acidification was carried out at 37°C for 4 days.

[0032] 3) Solid-liquid separation is carried out after acidification, and the acidified liquid obtained by acidification is used as a fermentation carbon source of PHA.

Embodiment 3

[0034] 1) After the wheat straw is pretreated by steam explosion, it is dried and crushed to 20 mesh;

[0035] 2) Using the domesticated activated sludge as the seed sludge, mix the steam explosion pretreated wheat straw with the activated sludge mass ratio of 5:1 evenly, then add water to adjust the solid-liquid ratio to 1:8, and adjust the pH to After 6.0, nitrogen gas was introduced into the reaction system, and anaerobic acidification was carried out at 37°C for 5 days.

[0036] 3) Solid-liquid separation is carried out after acidification, and the acidification solution is used as a fermentation carbon source for cutinase.

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Abstract

The invention belongs to the field of lignocellulose biomass pretreatment and application thereof, and particularly relates to a method for preparing a fermentation carbon source by hydrolysis and acidification of biomass raw materials and application thereof. In the conventional methods for biotransformation by using active sludge, the active sludge is directly adopted for anaerobic digestion, but the organic substance content of the active sludge is low, so the concentration of the acidified organic acid is too low, then the fermentation efficiency is low, and the organic acid is difficultly used as an effective carbon source. The domesticated active sludge is used as seed sludge in the method, and the method comprises the following steps of: mixing proper pretreated lignocellulose raw materials and the active sludge uniformly in a certain mass ratio in a reactor, regulating the solid-liquid ratio and the pH value by adding water, then introducing nitrogen into the reactor, performing anaerobic acidification, and separating the acidified solution to obtain the fermentation carbon source. The method can realize clean and efficient utilization of the lignocellulose raw materials, and has the advantages of high hydrolyzed and acidified organic acid concentration, short domestication time, reduction of production cost of the microbial fermentation carbon source and the like.

Description

technical field [0001] The invention belongs to the field of lignocellulosic biomass pretreatment and its application, and in particular relates to a method for preparing fermentation carbon source by hydrolyzing and acidifying biomass raw materials. Background technique [0002] With the depletion of fossil raw materials and people's concern about environmental protection, the search for renewable resources has become the focus of attention of all countries in the world. Biomass resources are one of the most abundant renewable resources in the world, which can be processed into various bio-based products, such as bio-based energy, bio-based chemicals and bio-based materials, through physical, chemical, biological and other transformation technologies. [0003] The hydrolysis and liquefaction of lignocellulosic raw materials has always been the limiting factor restricting its further conversion. Because lignocellulosic raw materials are composed of complex substances such a...

Claims

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Application Information

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IPC IPC(8): C12P1/00
Inventor 陈洪章邱卫华
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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