Method for promoting decomposition of straw lignocellulose

A technology of lignocellulose and straw, applied in the biological field, can solve the problems of high cost, insufficient utilization of straw resources, poor effect, etc., and achieve the effect of increasing the concentration of reducing sugar

Inactive Publication Date: 2011-09-07
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The effect of acid-base pretreatment is better, but the separation of acid-base after treatment is difficult, and the cost is too high, which is not conducive to mass application
Traditional mechanical pretreatment only changes the particles and cannot break the lignocellulose structure of the straw. The general upper limit of crushing is 300 meshes, which has little effect on the breaking of lignocellulose, so it cannot better promote the further development of lignocellulose. Decomposition, unable to make full use of straw resources
The effect of direct biological pretreatment is not good, it needs to consume a lot of cellulase, and the cost is very high

Method used

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  • Method for promoting decomposition of straw lignocellulose
  • Method for promoting decomposition of straw lignocellulose

Examples

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Effect test

Embodiment 1

[0019] A method to promote the decomposition of straw lignocellulose: After cutting the sorghum stalks, place them in an oven at 55°C for 7 hours, first use a crusher (manufactured by Tianjin Test Instrument Co., Ltd., model FY130) to coarsely crush to 160 mesh, and then Use a hanging nano ball mill (produced by Qinhuangdao Taiji Nano Co., Ltd., model MY-SQ-B) to superfinely pulverize for 4 hours, and the obtained straw particle size range is 330~420nm.

Embodiment 2

[0021] A method to promote the decomposition of straw lignocellulose: After cutting the straw into pieces, place it in an oven at 62°C for 5 hours, first crush it to 200 mesh with a crusher (manufactured by Tianjin Test Instrument Co., Ltd., model FY130), and then use it. Hanging type nano ball mill (produced by Qinhuangdao Taiji Nano Co., Ltd., model MY-SQ-B) can be superfinely pulverized for 6 hours, and the obtained straw particle size range is 330~420nm.

[0022] The sorghum stalks obtained in the above examples have a particle size range of nanometers (330~420nm). When using them for enzymatic hydrolysis, the concentration of reducing sugars produced is detailed in Figure 5 . Enzymatic hydrolysis reaction conditions: add 120mg of cellulase (Trichoderma viride, enzyme activity 3×10 per 100mL, pH 4.8 citric acid-sodium citrate buffer) 4 U·g -1 , Provided by Zhengzhou Boxing Biological Technology Co., Ltd.), then add 2g of the sorghum straw particles obtained in Example 2 to ea...

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Abstract

The invention relates to a method for promoting the decomposition of straw lignocellulose. The method comprises the following steps of: cutting up straws; placing in an oven at the temperature of between 55 and 65 DEG C for 5 to 7 hours; grinding by using a grinder until the particle size is 160 to 200 meshes; and grinding for 2 to 8 hours by using a nano ball mill. By the method, the structure of the straw lignocellulose can be relatively thoroughly broken, so that the particle sizes of the straws is more than the nano level, a subsequent enzymolysis reaction is facilitated, and the concentration of reducing sugar in the enzymolysis reaction is obviously improved and reaches 16mg.ml<-1>.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method capable of promoting the decomposition of straw lignocellulose. Background technique [0002] The crystallinity and lignification of natural cellulose raw materials hinder the conversion of lignocellulose into high-grade energy sources (such as ethanol and hydrogen), so pretreatment must be carried out before cellulose hydrolysis. Commonly used pretreatment methods include acid-base pretreatment, mechanical pretreatment and biological pretreatment. The effect of acid-base pretreatment is better, but the separation of acid-base after treatment is more difficult, and the cost is too high, which is not conducive to mass application. The traditional mechanical pretreatment is only a change in the particles and cannot break the lignocellulose structure of the straw. The general upper limit of pulverization is 300 mesh, which has little effect on lignocellulose, so it canno...

Claims

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Application Information

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IPC IPC(8): D21B1/06
Inventor 张全国杨群发胡建军周雪花冯宜鹏
Owner HENAN AGRICULTURAL UNIVERSITY
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