Glass-micropipe-based single nanopore sensor and manufacturing method thereof

Abstract

The invention discloses a glass-micropipe-based single nanopore sensor which comprises a glass micropipe, a single nanopore, two fluid chambers, a first voltage source, two Pt electrodes, an ammeter and a second voltage source, wherein the glass micropipe is provided with a protuberance at one end; the single nanopore is arranged in the protuberance; the two fluid chambers are respectively arranged at both ends of the single nanopore and connected with each other through the single nanopore; the first voltage source is used for applying voltages to the two fluid chambers; the two Pt electrodes are arranged on the protuberance and arranged at the upside and downside of the single nanopore; the ammeter is used for detecting voltage between the two Pt electrodes and both ends of the ammeter are respectively connected with the two Pt electrodes; the second voltage source is used for applying voltage to the two Pt electrodes; and the single nanopore and the fluid chambers are all provided with electrolyte. The sensor provided by the invention uses four electrodes to obtain radial current and axial current, so that the sensor disclosed by the invention can have higher sensitivity. Meanwhile, the method disclosed by the invention is simple and can greatly reduce the manufacturing cost of the sensor.

Description

technical field [0001] The invention relates to a device for detecting molecules and a manufacturing method thereof, in particular to a glass microtube-based single nanopore sensor and a manufacturing method thereof. Background technique [0002] At present, nanochannels mainly include natural nanochannels and solid nanochannels. The preparation of solid-state nanopores is mainly carried out on solid-state thin film materials. The methods include: ① ion beam engraving, ion beam sputtering to erode the porous membrane material to form a nanopore or stimulate the lateral transport of substances to shrink into a nanopore; ② micro-sculpture casting, first Use photolithography to make a master plate, and then deposit metal on PDMS to prepare nanopores; ③Sneak track etching, after the polymer film is irradiated by high-energy heavy ions, a sneak track is generated along the incident ion path, and then the potential ion track of the film is Perform chemical etching to form a porou...

AI Technical Summary

Problems solved by technology

However, current detection is to generate ion current by applying voltage in the axial direction, but applying voltage in the axial direction will limit its resolution and cannot judge the movement state of the measured medium in the hole.

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