Application of Honokiol to preparing drugs for preventing or treating intracranial space occupying lesion and intracranial tissues and organs inflammation
A honokiol, tissue and organ technology, applied in the field of preparation of drugs for preventing or treating intracranial space-occupying lesions and intracranial tissue and organ inflammation
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Embodiment 1
[0039] The preparation of embodiment one honokiol injection
[0040] Prepared a kind of intravenous injection preparation of honokiol, formula is: honokiol 0.2%, Cremophor EL (polyoxyethylene castor oil) 1.5%, ethanol 1.5%, vitamin C 0.2%, EDTA disodium salt 0.03% , the water balance.
[0041] Preparation method: Accurately weigh the drug, add Cremophor EL and ethanol, stir until the drug is completely dissolved, add the aqueous solution of vitamin C and EDTA disodium salt, stir evenly, add water for injection to the specified amount, stir evenly until clear, and dilute hydrochloric acid or Adjust the pH to neutral with dilute sodium hydroxide. Filtered, potted and sterilized.
Embodiment 2
[0042]Example 2 Honokiol Injection Penetrates Blood Brain Barrier and Blood Cerebrospinal Fluid Barrier Test after Intravenous Administration
[0043] Laboratory method: 30 male SD rats with a body weight of 220-260g (from the West China Animal Room of Sichuan University), 6 for the determination of drug concentration-time curves in plasma, and another 24 for 5 minutes, 10 Minutes, 60 minutes and 120 minutes four time points (6 rats for each time point) drug concentration test in the brain and cerebrospinal fluid. After 6 rats were anesthetized, they were fixed in a supine position, and the right common carotid artery was intubated; the tail vein was given honokiol 20 mg / kg body weight, and the injection was prepared according to the magnolol injection in Example 1. Blood was collected at 0 minutes, 5 minutes, 10 minutes, 15 minutes, 30 minutes, 45 minutes, 60 minutes, 90 minutes and 120 minutes, and centrifuged at low temperature to obtain plasma. HPLC analysis drug concentr...
Embodiment 3
[0053] Example 3 In vitro proliferation test of honokiol inhibiting mouse 9L gliosarcoma cells and human U251 glioma cells
[0054] Laboratory method: Mouse 9L gliosarcoma cells and human U251 glioma cells were cultured in 96-well plates, and the plating density was 5×10 4 After the cells adhered to the wall, honokiol of different concentrations (0-32 μg / mL) was added, and the fresh DMEM medium was used as a blank. After incubation for 24 hours, the absorbance value was measured at 570 nm by the MTT method, and the IC was calculated. 50 . see results image 3 (A, C), the IC of honokiol on mouse 9L gliosarcoma cells 50 was 15.61μg / mL, while IC for human U251 glioma cells 50 It was 16.38 μg / mL.
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