Nucleotide sequence and detection kit for detecting bovine enterovirus and foot-and-mouth disease virus

Abstract

The invention discloses a nucleotide sequence for detecting bovine enterovirus and foot-and-mouth disease virus, and the nucleotide sequence for detecting the bovine enterovirus and the foot-and-mouth disease virus is as shown in SEQ (sequence) ID (identity) NO: 1 to SEQ ID NO: 4 in a sequence table, wherein the SEQ ID NO: 1 and the SEQ ID NO: 2 are a sense primer and an antisense primer for detecting the bovine enterovirus respectively; and the SEQ ID NO: 3 and the SEQ ID NO: 4 are the sense primer and the antisense primer for detecting the foot-and-mouth disease virus respectively. The invention further discloses a kit containing the primers for detecting the bovine enterovirus and the foot-and-mouth disease virus. The kit has the characteristics of quickness, sensitiveness, specificity, stability and good repeatability.

Description

technical field

[0001] The invention relates to a nucleotide sequence and a detection kit for detecting bovine enterovirus and foot-and-mouth disease virus, especially a detection nucleotide sequence and a kit for multiplex RT-PCR detection of bovine enterovirus and foot-and-mouth disease virus, not only applicable to animals The accurate detection of tissue samples can also be applied to the detection of living animal samples (throat swabs, anal swabs, feces, serum, herpes skin, herpes fluid, conjunctival secretions, etc.), and can be used for domestic screening of bovine enteroviruses. Inspection and entry-exit quarantine, as well as simultaneous detection and differential diagnosis of bovine enterovirus and foot-and-mouth disease virus, etc., belong to the field of animal disease detection, inspection and quarantine. Background technique

[0002] Both bovine enterovirus and foot-and-mouth disease virus are members of the Picornaviridae family. Picornavirus is an extremel...