Method for preparing pomegranate peel polyphenol liposome by reverse evaporation method
A technology of reverse evaporation method and pomegranate peel, which is applied in the direction of liposome delivery, pharmaceutical formulations, medical preparations of non-active ingredients, etc., can solve problems such as poor stability, loss of physiologically active functions and nutritional value, and limited application. good quality effect
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Embodiment 1
[0017] Put 308 mg of soybean lecithin and 100 mg of cholesterol in a pear-shaped bottle, add 10 mL of chloroform to dissolve soybean lecithin and cholesterol, add 2.5 mL of pomegranate peel polyphenol phosphate buffer solution with a concentration of 1 mg / mL, and ultrasonicate for 5 to 8 minutes, and the mixture Form a homogeneous single-phase system, remove chloroform by rotary evaporation at 40°C, make the liposomes form a film on the bottle wall, dissolve the lipid film with 30 mL of phosphate buffer solution with a pH value of 6.17, fully hydrate, and filter with a filter membrane with a pore size of 0.45 μm , prepared into pomegranate peel polyphenol liposomes, and its encapsulation efficiency was 54.87%.
[0018] The pomegranate peel polyphenol phosphate buffer solution with a concentration of 1 mg / mL in this example is prepared from pomegranate peel dry powder and a phosphate buffer solution with a pH value of 6.5.
Embodiment 2
[0020] Put 300 mg of soybean lecithin and 100 mg of cholesterol in a pear-shaped bottle, add 7.5 mL of chloroform to dissolve soybean lecithin and cholesterol, add 2.5 mL of pomegranate peel polyphenol phosphate buffer solution with a concentration of 1 mg / mL, and ultrasonicate for 5 to 8 minutes. The mixture forms a homogeneous single-phase system. Remove chloroform by rotary evaporation at 40°C to make the liposomes form a film on the bottle wall. Dissolve the lipid film with 30mL of phosphate buffer solution with a pH value of 6.5, fully hydrate, and use a filter membrane with a pore size of 0.45μm After filtering, the pomegranate peel polyphenol liposome was prepared, and the encapsulation efficiency was 53.24%.
[0021] The preparation method of the pomegranate peel polyphenol phosphate buffer solution of this embodiment is the same as that of Example 1.
Embodiment 3
[0023] Put 100 mg of soybean lecithin and 100 mg of cholesterol in a pear-shaped bottle, add 2.5 mL of chloroform to dissolve soybean lecithin and cholesterol, add 2.5 mL of pomegranate peel polyphenol phosphate buffer solution with a concentration of 1 mg / mL, and ultrasonicate for 5 to 8 minutes. The mixture forms a homogeneous single-phase system, chloroform is removed by rotary evaporation at 40°C, and the liposomes are formed into a film on the bottle wall, and the lipid film is dissolved with 30 mL of phosphate buffer solution with a pH value of 4.5, fully hydrated, and filtered through a filter membrane with a pore size of 0.45 μm Filtrate to prepare pomegranate peel polyphenol liposome.
[0024] The preparation method of the pomegranate peel polyphenol phosphate buffer solution of this embodiment is the same as that of Example 1.
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