Full-automatic microbial staining film production equipment and staining method

Abstract

The invention discloses a fully automatic microbial dyeing film making device and a dyeing method. There is a washing pool in the inner cavity of the cabinet, and a washing pool lifting mechanism is connected between the motor and the washing pool. The inlet and outlet pipe solenoid valves are set separately; the inner wall of the washing pool is equipped with a miniature liquid level sensor; the top of the inner cavity of the cabinet is equipped with a ring-shaped ultraviolet lamp tube and a pair of dust collectors are installed on the inner surface, and the middle of the pair of dust collectors is hollow. Structured corona electrode, directly above the washing pool is a coaxially centered turntable glass slide platform and a porous dripping tray; the bottom of each funnel-shaped reagent container is connected to the output pipeline equipped with a miniature solenoid valve; Sterilize, ventilate and remove dust in the cabinet; write the dyeing scheme and steps into the automatic controller in advance, completely simulate the whole process of dyeing and film production, realize different dyeing steps through programming, and accurately control the time of each dyeing and washing to achieve Uniform and high-quality staining results.

Description

technical field [0001] The invention relates to a dyeing and film-making technology, in particular to the dyeing and film-making of microorganisms for detecting high-precision microorganisms. Background technique [0002] The current traditional microbial detection method is to observe and sample the microorganisms under an optical microscope or a fluorescent microscope after Gram staining or capsular staining. Because the difference in refractive index between undyed microorganisms and the surrounding environment is very small, it is extremely difficult to observe under a microscope. After staining, the microorganisms are in sharp contrast with the environment, and the morphology, arrangement and certain structural features of the microorganisms can be clearly observed for classification and activity detection. [0003] At present, most of the dyeing and film production work is done manually, and the whole dyeing process lasts about ten minutes, which consumes a lot of man...

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Problems solved by technology

[0003]At present, most of the dyeing and film production work is done manually, and the whole dyeing process lasts about ten minutes, which consumes a lot of manpower and material resources. In addition, if There are many observation samples, long dyeing time, and increased work fatigue, which will inevitably affect the standardization of operation, resulting in poor quality of film production and affecting detection accuracy

Chinese Patent No. CN00106925.X discloses an automatic staining device for the staining of microscopic inspection objects placed on slides. The device is equipped with a plurality of reagent containers arranged in front and back. The basket is loaded with multiple slides, and the transport basket is simultaneously lifted from the corresponding reagent container by an electric transport mechanism with a lifting device and transported one step forward into the adjacent reagent container, although this staining device realizes slides Batch dyeing greatly improves the dyeing efficiency, but the structure of the device is complicated, and the device does not contain measures such as sterilization and dust removal, which makes the device not suitable for high-precision microbial detection such as bacteria. In addition, the device is not fully automatic , there is still a certain amount of manual work before use.

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