Method for preparing acellular dermal matrix by utilizing ultrasonic wave

An acellular dermis and ultrasonic technology, applied in the field of medical skin tissue engineering, can solve the problems of long processing time, multi-chemical substances, complicated preparation process, etc., and achieve the effects of simple and practical preparation process, short production cycle and environmental friendliness
CN102225218AInactive Publication Date: 2011-10-26THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Publication Date
2011-10-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing an acellular dermal matrix by utilizing ultrasonic wave. The method comprises the following steps of: removing hair from peeled mammal skin; disinfecting the skin with disinfectant, and preparing reticular-layer derma by adopting a mechanical method under the sterile condition; oscillating the derma in hyposmotic solution for 0.5-12 hours, and then oscillating the derma in hyperosmotic solution for 0.5-12 hours; repeating the steps 1-6 times; placing the derma into washing agent and processing with an ultrasonic cleaner for 12-48 hours; and washing with sterile phosphate buffer solution 1-6 times, and disinfecting with ethylene oxide to obtain the acellular dermal matrix. According to the method disclosed by the invention, the preparation process is simple and practical, cells can be thoroughly removed, and the production period is short; and the method is environmentally-friendly, has low cost and is applicable to large-scale operation.
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Description

technical field

[0001] The invention relates to the field of medical skin tissue engineering, in particular to a preparation method of heterogeneous acellular dermal matrix. Background technique

[0002] Skin defects caused by burns, wounds, war injuries and other factors often leave scar hyperplasia and contracture deformity, which seriously affect the appearance and function. Loewe was the first to prove that dermal tissue can enhance the toughness of grafted skin, inhibit the growth of granulation tissue and scar formation, and reduce wound contracture degree. If the reconstruction of dermal structure is combined with other measures to promote wound healing, it can effectively reduce the formation of scars and improve the quality of wound healing.

[0003] The key problem of the skin all the time is immune rejection, which is mainly derived from cellular components such as epidermal cells, fibroblasts, and endothelial cells. Acellular Dermal Matrix (ADM) is a dermal sub...

Claims

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