Litsea cubeba pers asexual quick propagation and seedling breeding method

A technology for fast-growing seedlings and Radix japonica, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of slow growth and low survival rate of leaves, and achieves improved yield and quality, and forest growth. Good, cost-reducing effect

Inactive Publication Date: 2011-11-02
GUANGXI FORESTRY RES INST
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Problems solved by technology

The survival rate of the leaves is rela

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  • Litsea cubeba pers asexual quick propagation and seedling breeding method

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Embodiment 1

[0040] The rooting time of Litsea Cubeba subculture buds in vitro is April. Use the bud organ of a single plant of Litsea cubeba fruit output ≥ 40kg as explants, sterilize to obtain aseptic buds, transfer the aseptic buds to the subculture medium for proliferation and strong seedling cultivation. The subculture medium consists of MSC ( MS+Ca(NO 3 ) 2 380mg / L+H 3 BO 3 8.2mg / L)+BA 1.6mg / L+IAA 0.1mg / L+VC 10mg / L, the strong subculture medium was composed of MSC(MS+Ca(NO 3 ) 2 380mg / L+H 3 BO 3 8.2mg / L)+BA 0.6mg / L+IAA0.3mg / L+VC 15mg / L. The culture medium was divided into 500ml Erlenmeyer flasks, each bottle was 65ml, sealed with cotton balls, and then wrapped with kraft paper to wrap the cotton balls and the mouth of the bottle. 2 Under certain conditions, use a high-pressure steam sterilizer to sterilize for 15 minutes before use. In the aseptic operation room, inoculate the vigorous shoots on the new subculture medium in the form of 5 plants per cluster and 6 clusters per...

Embodiment 2

[0043] The rooting time of Litsea Cubeba Tissue Culture subculture bud outside test tube is October. Use the bud organ of a single plant of Litsea cubeba fruit output ≥ 40kg as explants, sterilize to obtain aseptic buds, transfer the aseptic buds to the subculture medium for multiplication and strong seedling cultivation. The subculture medium was composed of MSC (MS+Ca(NO 3 ) 2 380mg / L+H 3 BO 3 8.2mg / L)+BA 1.6mg / L+IAA 0.1mg / L+VC 10mg / L, subculture strong seedling medium is composed of MSC(MS+Ca(NO 3 ) 2 380mg / L+H 3 BO 3 8.2mg / L)+BA 0.6mg / L+IAA 0.3mg / L+VC 15mg / L. The culture medium was divided into 500ml Erlenmeyer flasks, each bottle was 65ml, sealed with cotton balls, and then wrapped with kraft paper to wrap the cotton balls and the mouth of the bottle. 2Under certain conditions, use a high-pressure steam sterilizer to sterilize for 15 minutes before use. In the aseptic operation room, inoculate the vigorously growing shoots on the new subculture medium in the form...

Embodiment 3

[0046] Litsea Cubeba seedlings are cut in November. Choose an area with flat terrain, convenient transportation, and good water supply and drainage as the nursery; after confirming the nursery, cultivate the land manually or mechanically, with a depth of 30cm, and raise the furrow. According to the specifications of about 1000cm in length, 120cm in width, 30cm in height, and drainage ditch width of 50cm, establish a soil seedbed or an elevated sandbed 50cm above the ground; Root water, cut off the top at 15cm from the ground in 30 days to promote germination; seedlings resume growth, start topdressing when 3 tender leaves grow, topdressing once every 20 days, compound fertilizer, water and fertilizer concentration 0.2-0.5%, depending on the growth of seedlings and climate Changes in the state, the concentration of fertilization from thin to thick, and the number of times from more to less, should be fertilized in time after collecting the spikes.

[0047] At 9 o'clock in the ...

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Abstract

The invention discloses a litsea cubeba pers asexual quick propagation and seedling breeding method. The method is a method which comprises the following steps that: rooting is performed outside a litsea cubeba pers tissue cultured sub-generation bud test tube; a litsea cubeba pers high-quality clonal tissue cultured sub-generation bud serves as a cutting shoot; seedling breeding is performed outside a litsea cubeba pers high-quality strain tissue cultured sub-generation bud test tube or through tissue cultured seedling young stem cutting seedling cultivation; MSC (MS+2,380mg/L of Ca(NO3) +38.2mg/L of H3BO)+ 1.6mg/L of BA+0.1mg/L of IAA+10mg/L of VC serves as a sub-generation multiplication culture medium; MSC (MS+2,380mg/L of Ca(NO3) +38.2mg/L of H3BO)+ 0.6mg/L of BA+0.3mg/L of IAA+15mg/L of VC serves as a sub-generation strong seedling culture medium; and yellow soil serves as a substrate; and a litsea cubeba pers sub-generation bud cutting seedling or a litsea cubeba pers tissue cultured young stem cutting seedling is cultured under the culture condition of irradiation of natural scattered light. The cutting survival rate of the sub-generation bud is 85 to 95 percent and the cutting survival rate of the young stem is 80 to 90 percent. The method has the advantages of high reproductive rate, large seedling culture amount, simple method, easiness in operation and low material consumption. The excellent genetic characteristics of a mother strain can be kept in the filial generation; and the problem on asexual seedling breeding of high-quality litsea cubeba pers is solved.

Description

technical field [0001] The invention belongs to the technical field of plant asexual rapid propagation, and relates to a method for raising litsea cubeba seedlings by asexual rapid propagation. Background technique [0002] Litsea Cubeba (Lour.), also known as Litsea Cubeba (Lour.), also known as Litsea Cubeba (Lour.), belongs to the genus Litsea Lam of Lauraceae (Lauraceae). Litsea cubeba oil can be distilled from the fruit and bark, and the mature fruit has the highest oil content. Litsea cubeba oil contains citral, higher alcohols, and organic acids, and the content of citral is as high as 70%. It is the preparation of essence, fragrance and synthetic vitamin A , E, the main raw material, can also be directly used for insecticide sterilization, has been China's largest export earnings of natural plant essential oil. Litsea Cubeba is mainly distributed in China's Zhejiang, Fujian, Hunan, Hubei, Jiangxi, Guangdong, Guangxi, Yunnan, Guizhou, Taiwan and other provinces (regi...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H4/00
CPCY02A40/22Y02P60/40
Inventor 蔡玲吴幼媚黄金使王以红覃子海
Owner GUANGXI FORESTRY RES INST
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