Gel microsphere capable of being injected with rifamycinoid antibiotics and preparation method thereof
A technology of rifamycin and gel microspheres, which is applied in the directions of antibacterial drugs, pharmaceutical formulations, medical preparations with inactive ingredients, etc. It can achieve the effect of good slow-release and controlled-release effect, low cost, and uniform drug dispersion.
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Embodiment 1
[0031] 1. Preparation of Injectable Rifapentine Gel Microspheres
[0032] (1) Dissolve 4.5 g of rifapentine in 20 mL of dimethyl sulfoxide solvent to obtain rifapentine dimethyl sulfoxide solution (solution A).
[0033] (2) Weigh 2.2g of polyethylene succinate and dissolve in solution A to obtain solution B.
[0034] (3) Weigh 1.8 g of sodium alginate and dissolve it in 100 mL of distilled water to obtain a 1.8% sodium alginate solution (solution C).
[0035] (4) Add solution B dropwise to 400 mL of distilled water, and stir continuously at 700 r / min to form a precipitate of rifapentine solid dispersion. After centrifugal precipitation, the supernatant was discarded, and 100 mL of distilled water was added to wash the solid dispersion precipitate once.
[0036] (5) The rifapentine solid dispersion obtained in the previous step was precipitated and dried, weighed, and suspended in 100 mL of solution C to obtain rifapentine sodium alginate glue.
[0037] (6) Take 7.5g of anhy...
Embodiment 2
[0044] 1. Preparation of Injectable Rifabutin Gel Microspheres
[0045] (1) Dissolve 3.2 g of rifabutin in 20 mL of dimethyl sulfoxide solvent to obtain rifabutin dimethyl sulfoxide solution (solution A).
[0046] (2) Weigh 2.0 g polyethylene succinate and dissolve in solution A to obtain solution B.
[0047] (3) Weigh 1.8 g of sodium alginate and dissolve it in 100 mL of distilled water to obtain a 1.8% sodium alginate solution (solution C).
[0048] (4) Add solution B dropwise to 400 mL of distilled water, and stir continuously at 700 r / min to form a solid dispersion of rifabutin. After centrifugal precipitation, the supernatant was discarded, and 100 mL of distilled water was added to wash the solid dispersion precipitate once.
[0049] (5) Suspend the rifabutin solid dispersion precipitate obtained in the previous step in 100 mL of solution C to obtain rifabutin sodium alginate glue.
[0050] (6) Take 7.5g of anhydrous calcium chloride and dissolve in 500mL distilled wa...
Embodiment 3
[0057] 1. Preparation of Injectable Rifapentine Gel Microspheres
[0058] (1) Dissolve 2.5 g of rifapentine in 20 mL of dimethyl sulfoxide solvent to obtain rifapentine dimethyl sulfoxide solution (solution A).
[0059] (2) Weigh 2g of polyethylene succinate and dissolve in solution A to obtain solution B.
[0060] (3) Weigh 1.8 g of sodium alginate and dissolve it in 100 mL of distilled water to obtain a 1.8% sodium alginate solution (solution C).
[0061] (4) Add solution B dropwise to 400 mL of distilled water, and stir continuously at 700 r / min to form a solid dispersion of rifapentine. After centrifugal precipitation, the supernatant was discarded, and 100 mL of distilled water was added to wash the solid dispersion precipitate once.
[0062] (5) Suspend the rifapentine solid dispersion precipitate obtained in the previous step in 100 mL of solution C to obtain rifapentine sodium alginate glue.
[0063] (6) Take 5g of anhydrous calcium chloride and dissolve it in 500mL...
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