Cicada extract shampoo and preparation thereof
A technology of extract and shampoo, which is applied in the field of cicada flower extract shampoo and its preparation, can solve the problems of inability to achieve ultra-fine cell wall breaking, waste, cost increase, etc.
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Embodiment 1
[0070] The acquisition of embodiment 1 Paecilomyces cicadae fermentation product
[0071] 1. Preparation and cultivation of strains:
[0072] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;
[0073] ①Preparation of slant seeds: wild cicadas collected from the source of Sanjiang in Yunnan Province were obtained through tissue separation and purification, and after transferring to tubes, they were cultured at 22°C for 5 days, and selected for future use;
[0074] ②Preparation of the primary seed: put the potato sucrose culture solution into the Erlenmeyer flask, put it in a sterilizing pot and sterilize it under the pressure of 0.15MPa for 30min, and put the spare slanted seed into the Erlenmeyer flask under aseptic condition after cooling , cultivated for 5 days at 22°C with an oscillation frequency of 130r / min, until a large number of mycelium balls appeared in the Erlenmeyer flask and then used for later use; ...
Embodiment 2
[0085] The acquisition of embodiment 2 Paecilomyces cicadae fermentation product
[0086] 1. Preparation and cultivation of strains:
[0087] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;
[0088] ①Preparation of slant seeds: wild cicadae collected from the source of Sanjiang in Yunnan Province was obtained through tissue separation and purification, and after transferring to tubes, cultured at 25°C for 7 days, and selected for future use;
[0089] ②Preparation of the primary species: put the potato sucrose culture solution into a conical flask, sterilize it in a sterilizing pot for 30 minutes under a pressure of 0.15MPa, put it into a conical flask after cooling, and place it at 25°C with an oscillation frequency of 150r / Cultivate for 3 days under the condition of min, until a large number of mycelium balls appear in the Erlenmeyer flask for later use;
[0090] Potato sucrose agar medium and potato sucrose...
Embodiment 3
[0095] The acquisition of embodiment 3 Paecilomyces cicadae fermentation product
[0096] 1. Preparation and cultivation of strains:
[0097] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;
[0098] ①Preparation of slant seeds: wild cicadas collected from the source of Sanjiang in Yunnan Province were obtained through tissue separation and purification, and after transferring to tubes, they were cultured at 22°C for 5 days, and selected for future use;
[0099]②Preparation of the primary species: put the potato sucrose culture solution into a conical flask, put it in a sterilizer for 30 minutes under a pressure of 0.15 MPa, put it into a conical flask after cooling, and place it at 23°C with an oscillation frequency of 130r / Cultivate under the condition of 5 min for 5 days, until a large number of mycelium balls appear in the Erlenmeyer flask for subsequent use;
[0100] Potato sucrose agar medium and potato ...
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